Charge and interfacial behavior of short side-chain heavily glycosylated porcine stomach mucin.
Charge and interfacial behavior of short side-chain heavily glycosylated porcine stomach mucin.
复制标题
短侧链重糖基化猪胃粘蛋白的电荷和界面行为。
DOI：
10.1021/bm700721c
复制
发表时间：
2007
影响因子：
6.2
通讯作者：
Yakubov GE
中科院分区：
文献类型：
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作者：
Yakubov GE
目前公认的MUC家族高分子量胃粘蛋白的模型是它们在本体溶液中采用多分散螺旋构象。我们开发了这个模型，使用良好的特点，高度纯化的猪胃粘蛋白，并检查分子的电荷和界面吸附。“Orthana”粘蛋白具有短的侧链、低水平的唾液酸残基，并且包括可负责粘蛋白的自聚合的微量胱氨酸残基。原子力显微镜和透射电子显微镜被用来检查粘蛋白的界面行为，并清楚地表明粘蛋白分子内的离散的球形亚基的存在，与静态光散射，动态光散射，和微电子电位测量的大小一致。此外，图像显示梳子是用串上的珠子组装的;菊花链模型。Zeta电位测量确定了粘蛋白分子的聚两性电解质性质，其用于解释它们在类似电荷表面上的吸附行为。
The current accepted model for high-molecular-weight gastric mucins of the MUC family is that they adopt a polydisperse coil conformation in bulk solutions. We develop this model using well-characterized highly purified porcine gastric mucin and examine the molecules’ charge and interfacial adsorption. “Orthana” mucin has short side-chains, low levels of sialic acid residues, and includes minute amounts of cystine residues that can be responsible for the self-polymerization of mucin. Atomic force microscopy and transmission electron microscopy are used to examine the interfacial behavior of the mucin and clearly demonstrate the existence of discrete spherical subunits within the mucin molecules, with sizes in agreement with static light scattering, dynamic light scattering, and ζ potential measurements. Furthermore images indicate the combs are assembled with a beads on a string conformation; the daisy chain model. Zeta potential measurements establish the polyampholyte nature of the mucin molecules, which is used to explain their adsorption behavior on similarly charged surfaces.