Analysis of paroxetine, fluoxetine and norfluoxetine in fish tissues using pressurized liquid extraction, mixed mode solid phase extraction cleanup and liquid chromatography-tandem mass spectrometry
Analysis of paroxetine, fluoxetine and norfluoxetine in fish tissues using pressurized liquid extraction, mixed mode solid phase extraction cleanup and liquid chromatography-tandem mass spectrometry
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DOI：
10.1016/j.chroma.2007.06.014
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发表时间：
2007-09-07
影响因子：
4.1
通讯作者：
Metcalfe, Chris D.
中科院分区：
文献类型：
--
作者：
Chu, Shaogang;Metcalfe, Chris D.
研究表明，由于城市污水处理厂向地表水排放药物，氟西汀等选择性5-羟色胺再摄取抑制剂(SSRI)在鱼的组织中积累。本研究建立了大气压化学电离-串联质谱仪(LC-AFCI-MS/MS)测定鱼组织中帕罗西汀、氟西汀及其活性代谢物去氟西汀残留量的分析方法。样品制备过程包括加压液体萃取(PLE)提取组织，然后在混合模式固相萃取柱(SPE)上净化，OASIS(R)NICK采用优化的方法，减少了基质干扰，回收率为85%。通过分析添加的鱼组织，测定帕罗西汀、氟西汀和去氟西汀的定量限(LOQ)分别为0.24、0.07和0.14 ng/g湿重。该方法成功地应用于加拿大安大略省汉密尔顿港的鱼类样品分析，汉密尔顿港是安大略湖的一个城市化和工业化的海湾。这些分析表明，这三种分析物在鱼类组织中的浓度高达约1微克/公斤湿重。(C)2007 Elsevier B.V.保留所有权利。
shown that selective serotonin reuptake inhibitors (SSRIs) such as fluoxetine are accumulated in the tissues of fish as a result of discharges of pharmaceuticals into surface waters from municipal wastewater treatment plants. In this study, an analytical method based on liquid chromatography with atmospheric pressure chemical ionization and tandem mass spectrometry (LC-AFCI-MS/MS) was developed and validated for the determination of residues of paroxetine, fluoxetine and its active metabolite, norfluoxetine, in fish tissue. The procedure for sample preparation includes extraction of tissue by pressurized liquid extraction (PLE), followed by cleanup on a mixed-mode solid phase extraction (SPE) cartridge, Oasis (R) NICK With the optimized method, matrix interferences were reduced and recoveries >85% were obtained. The limits of quantitation (LOQ) determined by analysis of spiked fish tissue were 0.24, 0.07, and 0.14 ng/g wet weight for paroxetine, fluoxetine and norfluoxetine, respectively. This method was successfully applied to the analysis of samples of fish collected from Hamilton Harbour in Ontario, Canada, which is an urbanized and industrialized embayment of Lake Ontario. These analyses showed that the three analytes were present in fish tissues at concentrations up to approximately 1 mu g/kg wet weight. (C) 2007 Elsevier B.V. All rights reserved.