Emerging tendency towards autoimmune process in major depressive patients: A novel insight from Th17 cells
Emerging tendency towards autoimmune process in major depressive patients: A novel insight from Th17 cells
复制标题
DOI：
10.1016/j.psychres.2010.10.029
复制
发表时间：
2011-07-30
影响因子：
11.3
通讯作者：
Sun, Yanhong
中科院分区：
文献类型：
--
作者：
Chen, Yili;Jiang, Tang;Sun, Yanhong
有证据表明，重度抑郁症 (MDD) 中出现了出现自身免疫的趋势。我们研究的目的是通过对 Th17 细胞的新认识来研究 MDD 中自身免疫过程的机制，Th17 细胞已被确定为自身免疫的重要激活剂。我们纳入了 40 名 MDD 患者和 30 名健康对照受试者。采用间接免疫荧光试验检测血清抗核抗体（ANA），结果显示患者组阳性率高于对照组。通过流式细胞术分析，抑郁症受试者外周Th17细胞数量显着增加，而T-reg细胞数量明显减少，与健康对照相比，显示出Th17/Treg比例失衡。我们还通过RT-PCR发现患者外周血淋巴细胞中RORγt（视黄酸相关孤儿受体-γt，Th17细胞的特异性转录因子）mRNA表达水平较高，并通过ELISA发现患者血清IL-17浓度较高。总之，我们的研究显示了 Th17 细胞在 MOD 患者自身免疫过程中的潜在作用，从而为 MDD 自身免疫倾向的现有证据做出了贡献。 (C) 2010 Elsevier Ireland Ltd. 保留所有权利。
Evidence indicates that there is an emerging tendency towards autoimmunity occuring in major depressive disorder (MDD). The aim of our study is to investigate the mechanism of autoimmune process in MDD from a novel insight of Th17 cells, which have been identified as the significant activators of autoimmunity. We included 40 patients with MDD and 30 healthy control subjects. An indirect immunofluorescence test was used for the detection of serum antinuclear antibodies (ANA), and revealed that the patient group was positive more frequently than the control group. By flow cytometric analysis, depressed subjects revealed a significant increase in peripheral Th17 cell number, and an obvious decrease in T-reg cell number, showing an imbalance of Th17/Treg ratio compared to healthy controls. We also found a higher level of ROR gamma t (retinoic acid-related orphan receptor-gamma t, the specific transcription factor of Th17 cell) mRNA expression in peripheral blood lymphocytes by RT-PCR and serum concentration of IL-17 by ELISA in patients. In conclusion, our study showed a potential role of Th17 cells in the autoimmune process in MOD patients, thus contributing to the existing evidence of autoimmune inclination in MDD. (C) 2010 Elsevier Ireland Ltd. All rights reserved.