Detection of Toll-like receptor 2 (TLR2) mutation in the lepromatous leprosy patients
Detection of Toll-like receptor 2 (TLR2) mutation in the lepromatous leprosy patients
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DOI：
10.1111/j.1574-695x.2001.tb01586.x
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发表时间：
2001-07-01
影响因子：
--
通讯作者：
Chae, GT
中科院分区：
文献类型：
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作者：
Kang, TJ;Chae, GT
Toll 样受体 2 (TLR2) 在分枝杆菌感染的免疫反应中至关重要，TLR2 的突变已被证明会导致对分枝杆菌严重感染的易感性。为了明确这一点，我们在 131 名受试者中筛选了 TLR2 的胞内结构域。对 45 名麻风病患者、41 名结核性麻风病 (TT) 患者和 45 名对照患者进行了研究。通过单链构象多态性检测，十名麻风病 (LL) 患者具有条带变异。 DNA 测序检测到 TLR2 起始密码子的核苷酸 2029 处存在 C 至 T 取代。该突变将在氨基酸残基 677（TLR2 的保守区域之一）处将 Arg 替换为 Trp。在我们的结果中，突变仅涉及 LL，而不涉及 TT 和对照。因此，我们认为 TLR2 胞内结构域的突变在 LL 易感性中发挥作用。 (C) 2001 年欧洲微生物学会联合会。由 Elsevier Science B.V. 出版。保留所有权利。
Toll-like receptor 2 (TLR2) is critical in the immune response to mycobacterial infections and the mutations in the TLR2 have been shown to confer the susceptibility to severe infection with mycobacteria. To define this, we screened the intracellular domain of TLR2 in 131 subjects. Groups of 45 lepromatous and 41 tuberculoid leprosy (TT) patients and 45 controls were investigated. Ten subjects among the lepromatous leprosy (LL) patients had a band variant detected by single-stranded conformational polymorphism. DNA sequencing detected a C to T substitution at nucleotide 2029 from the start codon of the TLR2. The mutation would substitute Arg to Trp at amino acid residue 677, one of the conserved regions of TLR2. In our results, the mutation was involved in only LL, not TT and control. Thus, we suggest that the mutation in the intracellular domain of TLR2 has a role in susceptibility to LL. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.