Discovering neuropeptides in Caenorhabditis elegans by two dimensional liquid chromatography and mass spectrometry
Discovering neuropeptides in Caenorhabditis elegans by two dimensional liquid chromatography and mass spectrometry
复制标题
DOI：
10.1016/j.bbrc.2005.07.044
复制
发表时间：
2005-09-16
影响因子：
3.1
通讯作者：
Schoofs, L
中科院分区：
文献类型：
--
作者：
Husson, SJ;Clynen, E;Schoofs, L
1998年秀丽隐杆线虫基因组测序计划的完成为线虫神经肽信号传导的复杂性提供了更多的见解。几个C。已从基因组数据库中预测了编码约250种肽的线虫神经肽前体基因。然而，人们不能推断出所有这些肽是否真的表达，也不可能预测所有的翻译后修饰。采用二维纳米液相色谱-串联质谱联用技术和数据库挖掘技术对混合阶段的秀丽隐杆线虫提取物进行了分析。这种肽组设置产生了21种来自以前预测的神经肽样蛋白（NLP）前体的肽和28种预测的FMRFamide相关肽。此外，我们能够测序11个全新的肽，这些肽来自9个肽前体，这些肽前体以前没有以任何方式预测或鉴定。一些已鉴定的肽显示出与其他无脊椎动物神经肽的深刻序列相似性，表明这些肽具有悠久的进化历史。(c)2005年爱思唯尔公司All rights reserved.
Completion of the Caenorhabditis elegans genome sequencing project in 1998 has provided more insight into the complexity of nematode neuropeptide signaling. Several C. elegans neuropeptide precursor genes, coding for approximately 250 peptides, have been predicted from the genomic database. One can, however, not deduce whether all these peptides are actually expressed, nor is it possible to predict all post-translational modifications. Using two dimensional nanoscale liquid chromatography combined with tandem mass spectrometry and database mining, we analyzed a mixed stage C elegans extract. This peptidomic setup yielded 21 peptides derived from formerly predicted neuropeptide-like protein (NLP) precursors and 28 predicted FMRFamide-related peptides. In addition, we were able to sequence 11 entirely novel peptides derived from nine peptide precursors that were not predicted or identified in any way previously. Some of the identified peptides display profound sequence similarities with neuropeptides from other invertebrates, indicating that these peptides have a long evolutionary history. (c) 2005 Elsevier Inc. All rights reserved.