THE 2 STAPHYLOCOCCAL BICOMPONENT TOXINS, LEUKOCIDIN AND GAMMA-HEMOLYSIN, SHARE ONE-COMPONENT IN COMMON
THE 2 STAPHYLOCOCCAL BICOMPONENT TOXINS, LEUKOCIDIN AND GAMMA-HEMOLYSIN, SHARE ONE-COMPONENT IN COMMON
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DOI：
10.1016/0014-5793(93)80611-w
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发表时间：
1993-04-19
期刊：
影响因子：
3.5
通讯作者：
IZAKI, K
中科院分区：
文献类型：
--
作者：
KAMIO, Y;RAHMAN, A;IZAKI, K
葡萄球菌双组分毒素，杀白细胞素和γ-溶血素，由两种蛋白质组分组成，即杀白细胞素的F和S以及γ-溶血素的HgammaI和HgammaII。在这项研究中，我们纯化的HgammaI和HgammaII同质的金黄色葡萄球菌RIMD 310925的培养基，并比较它们的性质与F和S从同一来源纯化。纯化的HgammaI的N-末端59-和C-末端2-残基氨基酸序列、表观分子量和等电点与F.在Ouchterlony双扩散试验中，使用抗F或抗HgammaI抗体，在F和HgammaI之间形成无突的融合线。F和HgammaII的协同作用引起人红细胞溶血，HgammaI与S协同作用，表现出杀白细胞素活性。我们的结论是，这两种毒素共享一个蛋白质组分（F = HgammaI）的共同和杀白细胞素和γ-溶血素的特异性活动确定S和HgammaII，分别。HgammaII的N端58个氨基酸序列与S.
Staphylococcal bi-component toxins, leukocidin and gamma-hemolysin, consist of two protein components, i.e. F and S for leukocidin and HgammaI and HgammaII for gamma-hemolysin. In this study we purified HgammaI and HgammaII to homogeneity from the culture medium of Staphylococcus aureus RIMD 310925 and compared their properties with those of F and S purified from the same source. The N-terminal 59- and C-terminal 2-residue amino acid sequences, apparent molecular mass, and isoelectric point of purified HgammaI were the same as those of F. In an Ouchterlony double diffusion test a fused line without spur was formed between F and HgammaI using either anti-F or anti-HgammaI antibodies. A synergistic action of F and HgammaII caused hemolysis of human red blood cells, and HgammaI acted synergistically with S to exhibit leukocidin activity. We conclude that the two toxins share one protein component (F = HgammaI) in common and leukocidin- and gamma-hemolysin-specific activities are determined by S and HgammaII, respectively. It is also reported that the N-terminal 58-residue sequence of HgammaII is 72% similar to the corresponding sequence of S.