Molecular Typing and Phenotype Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Blood in Taiwan
Molecular Typing and Phenotype Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Blood in Taiwan
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DOI：
10.1371/journal.pone.0030394
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发表时间：
2012-01-23
期刊：
影响因子：
3.7
通讯作者：
Lu, Jang-Jih
中科院分区：
文献类型：
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作者：
Wang, Wei-Yao;Chiueh, Tzong-Shi;Lu, Jang-Jih
背景：金黄色葡萄球菌引起多种严重感染，如菌血症和败血症。目前，台湾的金黄色葡萄球菌分离株中有60-80%是耐甲氧西林（MRSA）。研究表明，某些MRSA克隆体在世界范围内传播。本研究的目的是鉴定台湾地区的MRSA克隆，并将分离株的分子类型与其表型相关联。方法：收集9个医疗中心细菌性贫血患者157株MRSA。根据agr、SCCmec、MLST、spa和dru的多态性进行分型。表型包括潘通-瓦朗丁嗜白细胞素（pvl）、诱导型大环内酯-lincosamide-链状gramin B耐药（MLSBi）、万古霉素（VA）和达托霉素（DAP）最低抑制浓度（MIC）和超抗原毒素基因谱。两个连续样本之间的差异采用Mann-Whitney-U检验，两个分类变量之间的差异采用Fisher精确检验。结果：共分离到4个主要的MRSA克隆复合物CC1、CC5、CC8和CC59，其中CC1、CC5、CC8和CC59分别为4株、9株、111株和28株。这些克隆具有以下分子类型：CC1: SCCmecIV和ST573；CC5: SCCmecII和ST5；CC8: SCCmecIII、ST239、ST241, CC59: SCCmecIV、SCCmecV(T)、ST59、ST338。这些无性系毒素基因谱为CC1: sec-seg-(sei)-sell-selm-(seln)-selo；(CC5: sec-seg-sei-sell-selm) - seln -selp-tst1;CC8: sea-selk-selq, CC59: seb-selk-selq。SCCmecV(T)、ST59、spat437和dru11型多为pvl（+）型（13株），而SCCmecIII、ST239、spa t037、agrI和dru14型多药耐药（>= 4种抗菌素）相关（119株）
Background: Staphylococcus aureus causes a variety of severe infections such as bacteremia and sepsis. At present, 60-80% of S. aureus isolates from Taiwan are methicillin resistant (MRSA). It has been shown that certain MRSA clones circulate worldwide. The goals of this study were to identify MRSA clones in Taiwan and to correlate the molecular types of isolates with their phenotypes.Methods: A total of 157 MRSA isolates from bacteremic patients were collected from nine medical centers. They were typed based on polymorphisms in agr, SCCmec, MLST, spa, and dru. Phenotypes characterized included Panton-Valentine leucocidin (pvl), inducible macrolide-lincosamide-streptogramin B resistance (MLSBi), vancomycin (VA) and daptomycin (DAP) minimal inhibitory concentrations (MIC), and superantigenic toxin gene profiles. Difference between two consecutive samples was determined by Mann-Whitney-U test, and difference between two categorical variables was determined by Fisher's exact test.Results: Four major MRSA clone complexes CC1, CC5, CC8, and CC59 were found, including 4 CC1, 9 CC5, 111 CC8, and 28 CC59 isolates. These clones had the following molecular types: CC1: SCCmecIV and ST573; CC5: SCCmecII and ST5; CC8: SCCmecIII, ST239, and ST241, and CC59: SCCmecIV, SCCmecV(T), ST59, and ST338. The toxin gene profiles of these clones were CC1: sec-seg-(sei)-sell-selm-(seln)-selo; CC5: sec-seg-sei-sell-selm-(seln)-selp-tst1; CC8: sea-selk-selq, and CC59: seb-selk-selq. Most isolates with SCCmecV(T), ST59, spat437, and dru11 types were pvl(+) (13 isolates), while multidrug resistance (>= 4 antimicrobials) were associated with SCCmecIII, ST239, spa t037, agrI, and dru14 (119 isolates) (p