Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure
Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure
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DOI：
10.1080/14653240600621125
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发表时间：
2006-05-01
期刊：
影响因子：
4.5
通讯作者：
van Milligen, FJ
中科院分区：
文献类型：
--
作者：
Oedayrajsingh-Varma, MJ;van Ham, SM;van Milligen, FJ
背景脂肪组织含有易于分离的间质血管成分，为脂肪组织来源的间充质干细胞(ASC)提供了丰富的来源。这些ASC是组织工程的潜在细胞来源。我们研究了手术方式(切除、肿胀吸脂和超声辅助吸脂)是否影响ASC的产量和生长特性。方法采用极限稀释法测定ASC在间质血管中的比例。用流式细胞仪测定ASC的表型标志物，并研究ASC在培养过程中的生长动力学。ASC在软骨和成骨条件下培养，以确定其分化潜能。结果间质血管部分的存活细胞数既不受手术方式的影响，也不受脂肪组织来源的解剖部位的影响。三种手术方式后，培养的ASC均表达CD34(+)、CD31(-)、CD105(+)、CD166(+)、CD45(+)、CD90(+)ASC表型。然而，与切除相比，超声辅助脂肪抽吸术导致ASC增殖率降低，ASC群体倍增时间延长。ASC具有向软骨和成骨分化的潜能。结论脂肪组织采集手术方式对ASC的产量和生长特性有影响。对于组织工程的目的，切除和肿胀吸脂似乎比超声辅助吸脂更可取。
Background Adipose tissue contains a stromal vascular fraction that can be easily isolated and provides a rich source of adipose tissue-derived mesenchymal stem cells (ASC). These ASC are a potential source of cells for tissue engineering. We studied whether the yield and growth characteristics of ASC were affected by the type of surgical procedure used for adipose tissue harvesting, i.e. resection, tumescent liposuction and ultrasound-assisted liposuction.Methods Frequencies of ASC in the stromal vascular fraction were assessed in limiting dilution assays. The phenotypical marker profile of ASC was determined, using flow cytometry, and growth kinetics were investigated in culture. ASC were cultured under chondrogenic and osteogenic conditions to confirm their differentiation potential.Results The number of viable cells in the stromal vascular fraction was affected by neither the type of surgical procedure nor the anatomical site of the body from where the adipose tissue was harvested. After all three surgical procedures, cultured ASC did express a CD34(+) CD31(-) CD105(+) CD166(+) CD45(+) CD90(+) ASC phenotype. However, ultrasound-assisted liposuction resulted in a lower frequency of proliferating ASC, as well as a longer population doubling time of ASC, compared with resection. ASC demonstrated chondrogenic and osteogenic differentiation potential.Discussion We conclude that yield and growth characteristics of ASC are affected by the type of surgical procedure used for adipose tissue harvesting. Resection and tumescent liposuction seem to be preferable above ultrasound-assisted liposuction for tissue-engineering purposes.