Inter-laboratory validation of PCR-based HPV detection in pathology specimens
Inter-laboratory validation of PCR-based HPV detection in pathology specimens
复制标题
病理标本中基于 PCR 的 HPV 检测的实验室间验证
作者：
I. Petersen;C. Schewe;K. Schlüns;M. Dietel;N. Speich;C. Schmitt;M. Bollmann;K. Sotlar;B. Bültmann;M. Dours;B. Padberg;D. Zimmermann
病理标本中人乳头瘤病毒（HPV）的检测和分型变得越来越重要。在德国病理学会和德国病理学家专业协会病理学质量保证 (QuIP) 倡议的背景下，选择了四个在基于聚合酶链反应 (PCR) 的 HPV 检测方面拥有经验和专业知识的小组实验室来建立实验室间试验。第一步，这些实验室对自己的方法进行了内部测试，其中包括 DNA 测序、多重巢式 PCR 和杂交技术。每个小组研究所根据自己的方案对来自 39 个样品的材料（包括石蜡切片以及组织和质粒的 DNA 制剂）进行了评估。尽管方法不同，但仍实现了高度的实验室间可靠性。在本报告中，我们总结了结果。预先测试的样本可用于外部试验，并且可以通过柏林 provitro GmbH (http://www.provitro.de) 从指导研究所订购。补充数据可在线获取：http://pathologie-ccm.charite.de（标题为“Forschung”），其中包括 HPV 相关病变及其与特定病毒类型的潜在关联的基于网络的照片库。该计划旨在促进病理学分子 HPV 分析及其与形态变化的相关性的质量保证。
The detection and typing of human papilloma virus (HPV) in pathology specimens is gaining increasingly in importance. In the context of the initiative for quality assurance in pathology (QuIP) of the German Society of Pathology and the Professional Association of German Pathologists, four panel laboratories with experience and expertise in polymerase chain reaction (PCR)-based HPV detection were selected to establish an inter-laboratory trial. In a first step, these laboratories performed an internal testing of their own methodologies, which comprised DNA sequencing, multiplex nested PCR and hybridization techniques. Material from 39 samples including paraffin sections and DNA preparations of tissues and plasmids were evaluated by each panel institute according to their own protocols. Despite the different methodologies, a high degree of inter-laboratory reliability was achieved. In this report, we summarise the results. Pretested specimens are available for the external trail and can be ordered from the steering institute via provitro GmbH Berlin (http://www.provitro.de). Supplementary data are online available at http://pathologie-ccm.charite.de (rubric "Forschung"), which includes a web-based photo gallery of HPV-associated lesions and their potential association with specific virus types. The initiative is intended to foster the quality assurance of molecular HPV analysis in pathology and its correlation with morphological changes.
DOI：
10.1002/j.1460-2075.1989.tb08447.x
发表时间：
1989-10
期刊：
The EMBO Journal
影响因子：
--
作者：
Dieter R. Zimmermann;E. Ruoslahti
通讯作者：
Dieter R. Zimmermann;E. Ruoslahti
DOI：
--
发表时间：
1994-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Michael F. NasoS;Dieter R. Zimmermanng;Renato V. Iozzon
通讯作者：
Michael F. NasoS;Dieter R. Zimmermanng;Renato V. Iozzon