Y-box-binding protein 1 inhibits apoptosis and upregulates EGFR in colon cancer
Y-box-binding protein 1 inhibits apoptosis and upregulates EGFR in colon cancer
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DOI：
10.3892/or.2019.7038
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发表时间：
2019-05-01
期刊：
影响因子：
4.2
通讯作者：
Akagi, Yoshito
中科院分区：
文献类型：
--
作者：
Nagasu, Sachiko;Sudo, Tomoya;Akagi, Yoshito
Y-box-binding protein 1（YB-1）是一种DNA/RNA结合蛋白，是肿瘤发生过程中重要的转录和翻译因子。然而，YB-1在结直肠癌中的生物学功能和分子相关性尚不完全清楚。本研究的目的是确定YB-1表达的意义及其在结直肠癌中的生物学作用。在具有不同遗传背景的不同结肠癌细胞系中，在敲低YB-1表达后检查细胞增殖、迁移和凋亡。由于不同的结肠癌细胞系与特定的RAS/RAF基因突变下游的表皮生长因子受体（EGFR）的性质可能不同于野生型结直肠癌，这是至关重要的，以研究YB-1的RAS突变状态的作用。结果表明，抑制YB-1可降低细胞增殖（P
Y-box-binding protein 1 (YB-1) is a DNA/RNA-binding protein and an important transcription and translation factor in carcinogenesis. However, the biological function and molecular correlation of YB-1 in colorectal cancer are not fully understood. The aim of the present study was to determine the significance of YB-1 expression and its biological role in colorectal cancer. Cell proliferation, migration and apoptosis were examined upon knockdown of YB-1 expression in different colon cancer cell lines that had different genetic backgrounds. Since the properties of different colon cancer cell lines with specific RAS/RAF gene mutations downstream epidermal growth factor receptor (EGFR) may differ from wild-type colorectal cancer, it is critical to study the role of YB-1 with respect to the mutational status of RAS. The results indicated that the suppression of YB-1 decreased cell proliferation (P