Release of cytochrome c and decrease of cytochrome c oxidase in Bax-expressing yeast cells, and prevention of these effects by coexpression of Bcl-x(L)
Release of cytochrome c and decrease of cytochrome c oxidase in Bax-expressing yeast cells, and prevention of these effects by coexpression of Bcl-x(L)
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DOI：
10.1016/s0014-5793(97)01087-9
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发表时间：
1997-09-22
期刊：
影响因子：
3.5
通讯作者：
Guerin, M
中科院分区：
文献类型：
--
作者：
Manon, S;Chaudhuri, B;Guerin, M
表达促凋亡基因 Bax 或共表达 Bax 和抗凋亡基因 Bcl-x(L) 的酵母细胞的线粒体特征已在全细胞、分离线粒体和透化原生质球中进行了研究。研究发现，Bax 诱导的酵母细胞生长停滞与呼吸链中的两个缺陷有关：(i) 呼吸链末端酶细胞色素 c 氧化酶数量的减少，以及 (ii) 细胞色素 c 向胞质溶胶释放的急剧增加。线粒体内膜的其他成分（bc(1) 复合物和 F0F1-ATPase）不受影响。 Bcl-x(L) 的共表达几乎完全阻止了 Bax 的作用。令人惊讶的是，在酵母中获得的这些结果与在哺乳动物细胞中报道的类似观察结果相似，(C) 1997 年欧洲生化协会联合会。
The characteristics of mitochondria of yeast cells expressing the pro-apoptotic gene Bax or coexpressing Bax and the anti-apoptotic gene Bcl-x(L) have been investigated in whole cells, isolated mitochondria and permeabilized spheroplasts. It is found that Bax-induced growth arrest of yeast cells is related to two defects in the respiratory chain: (i) a decrease in the amount of cytochrome c oxidase, the terminal enzyme of the respiratory chain, and (ii) a dramatic increase in the release of cytochrome c to the cytosol. Other components of the inner mitochondrial membrane (bc(1) complex and F0F1-ATPase) are unaffected. Coexpression of Bcl-x(L) almost fully prevented the effect of Bax. Surprisingly, these results obtained in yeast parallel similar observations reported in mammalian cells, (C) 1997 Federation of European Biochemical Societies.