Multiple resistance to ACCase and AHAS-inhibiting herbicides in shortawn foxtail (Alopecurus aequalis Sobol.) from China
Multiple resistance to ACCase and AHAS-inhibiting herbicides in shortawn foxtail (Alopecurus aequalis Sobol.) from China
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DOI：
10.1016/j.pestbp.2015.04.006
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发表时间：
2015-10-01
影响因子：
4.7
通讯作者：
Wang, Jinxin
中科院分区：
文献类型：
--
作者：
Guo, Wenlei;Yuan, Guohui;Wang, Jinxin
看麦娘（Alopecurus aequalis）是危害我国冬小麦和油菜生产的重要禾本科杂草。精恶唑禾草灵和甲基二磺隆对安徽寿县小麦田的AHSX-1种群的赤霉病无防效。分子生物学分析表明，AHSX-1群体中存在ACC酶的Asp（2078）Gly突变和AHAS的Trp（574）Leu突变。分离并培养纯合植物直至种子成熟。在温室中使用F1代的纯化种子进行全株除草剂生物测定。剂量反应试验表明，AHSX-1对精恶唑禾草灵（RI = 275）和甲基磺隆（RI = 788）已产生了很高的抗性。为了确定对其他除草剂的敏感性，以每种除草剂的单一推荐剂量进行了测定。结果表明，AHSX-1种群对炔草酯、啶磺草胺和氟卡巴唑钠高抗，对精喹禾灵、烯草酮、稀禾啶和唑啉草酮中抗或高抗，对异丙隆和绿麦隆敏感。本研究首次报道了赤狐的Asp（2078）Gly突变，设计的两个dCAPS标记分别能快速检测赤狐ACCase和AHAS基因的Asp（2078）和Trp（574）突变。(C)2015爱思唯尔公司All rights reserved.
Shortawn foxtail (Alopecurus aequalis) is a troublesome grass weed infesting winter wheat and oilseed rape productions in China. Fenoxaprop-p-ethyl and mesosulfuron-methyl failed to control shortawn foxtail of AHSX-1 population collected from a wheat field in Shou County, Anhui province. Molecular analyses revealed that Asp(2078)Gly mutation of ACCase and Trp(574)Leu mutation of AHAS were present in plants of the AHSX-1 population. The homozygous plants were isolated and cultured until seed maturity. Whole-plant herbicide bioassays were conducted in the greenhouse using the purified seeds of F1 generation. Dose-response experiments showed that the AHSX-1 population has evolved a very high level resistance to fenoxaprop-p-ethyl (RI = 275) and mesosulfuron-methyl (RI = 788). To determine the sensitivity to other herbicides, assays were conducted at the single recommended rate of each herbicide. Based on the results, the AHSX-1 population was considered to be highly resistant to clodinafop-propargyl, pyroxsulam and flucarbazone-sodium, moderately or highly resistant to quizalofop-p-ethyl, clethodim, sethoxydim and pinoxaden, and susceptible to isoproturon and chlorotoluron. This is the first report of Asp(2078)Gly mutation in shortawn foxtail and the two robust dCAPS markers designed could quickly detect Asp(2078) and Trp(574) mutations in ACCase and AHAS gene of shortawn foxtail, respectively. (C) 2015 Elsevier Inc. All rights reserved.