Retrotransposon-mediated DELLA transcriptional reprograming underlies semi-dominant dwarfism in foxtail millet
Retrotransposon-mediated DELLA transcriptional reprograming underlies semi-dominant dwarfism in foxtail millet
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DOI：
10.1016/j.cj.2018.12.008
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发表时间：
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通讯作者：
Xianmin Diao
中科院分区：
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作者：
Meicheng Zhao;Hui Zhi;Xue Zhang;Guanqing Jia;Xianmin Diao
Retrotransposons account for a large proportion of the genome and genomic variation, and.play key roles in creating novel genes and diversifying the genome inmany eukaryotic species..Although retrotransposons are abundant in plants, their roles had been underestimated.because of a lack of research. Here, we characterized a gibberellin Acid (GA)-insensitive dwarf.mutant, 84133, in foxtail millet. Map-based cloning revealed a 5.5-kb Copia-like.retrotransposon insertion in DWARF1 (D1), which encodes a DELLA protein. Transcriptional.analysis showed that the Copia retrotransposonmediated the transcriptional reprogramming.of D1 leading to a novel N-terminal-deleted truncated DELLA transcript that was putatively.driven by Copia's LTR, namely D1-TT, and another chimeric transcript. The presence of D1-TT.was confirmed by protein immunodetection analysis. Furthermore, D1-TT protein was.resistant to GA3 treatment compared with the intact DELLA protein due to its inability to.interact with the GA receptor, SiGID1. Overexpression of D1-TT in foxtail millet resulted in.dwarf plants, confirming that it determines the dwarfismof 84133. Thus, our study documents.a rare instance of long terminal repeat (LTR) retrotransposon-mediated transcriptional.reprograming in the plant kingdom. These results shed light on the function of LTR.retrotransposons in generating new gene functions and genetic diversity.
