Degradation of de-esterified pctin/homogalacturonan by the polygalacturonase GhNSP is necessary for pollen exine formation and male fertility in cotton.
Degradation of de-esterified pctin/homogalacturonan by the polygalacturonase GhNSP is necessary for pollen exine formation and male fertility in cotton.
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多聚半乳糖醛酸酶 GhNSP 对脱酯化果胶/同型半乳糖醛酸的降解对于棉花花粉外壁的形成和雄性育性是必需的
DOI：
10.1111/pbi.13785
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发表时间：
2022-06
影响因子：
13.8
通讯作者：
Zhang, Xianlong
中科院分区：
文献类型：
--
作者：
Wu, Yuanlong;Li, Xiao;Li, Yanlong;Ma, Huanhuan;Chi, Huabin;Ma, Yizan;Yang, Jing;Xie, Sai;Zhang, Rui;Liu, Linying;Su, Xiaojun;Lv, Rongjie;Khan, Aamir Hamid;Kong, Jie;Guo, Xiaoping;Lindsey, Keith;Min, Ling;Zhang, Xianlong
关键词：
花粉壁外壁为雄配子体提供保护层，主要由孢粉素组成，其中包括脂肪酸衍生物和酚类。然而，外外壁的生化性质知之甚少。在这里，我们表明，棉花雄性不育系1355 A无刺花粉（GhNSP）突变导致有缺陷的外壁形成。GhNSP基因座通过基于图位的克隆进行鉴定，并通过遗传分析（共分离测试和使用CRISPR/Cas9系统的等位基因预测）进行确认。原位杂交结果表明，GhNSP在绒毡层中高表达。GhNSP编码一个与AtQRT 3同源的多聚半乳糖醛酸酶蛋白，这表明多聚半乳糖醛酸酶在花粉外壁形成中的功能。这些结果表明GhNSP与AtQRT 3在功能上存在差异，AtQRT 3具有分离小孢子的功能。生化分析表明，在发育阶段8，1355 A花药中脱酯化果胶的百分比显著增加。此外，使用去酯化和酯化的同型半乳糖醛酸聚糖（JIM 5和JIM 7）的抗体进行的免疫荧光研究表明，Ghnsp突变体在绒毡层和外壁中显示出大量的去酯化同型半乳糖醛酸聚糖，并伴有有缺陷的外壁形成。GhNSP的表征为多聚半乳糖醛酸酶和去酯化的同型半乳糖醛酸聚糖在花粉外壁形成中的作用提供了新的理解。
The pollen wall exine provides a protective layer for the male gametophyte and is largely composed of sporopollenin, which comprises fatty acid derivatives and phenolics. However, the biochemical nature of the external exine is poorly understood. Here, we show that the male sterile line 1355A of cotton mutated in NO SPINE POLLEN (GhNSP) leads to defective exine formation. The GhNSP locus was identified through map‐based cloning and confirmed by genetic analysis (co‐segregation test and allele prediction using the CRISPR/Cas9 system). In situ hybridization showed that GhNSP is highly expressed in tapetum. GhNSP encodes a polygalacturonase protein homologous to AtQRT3, which suggests a function for polygalacturonase in pollen exine formation. These results indicate that GhNSP is functionally different from AtQRT3, the latter has the function of microspore separation. Biochemical analysis showed that the percentage of de‐esterified pectin was significantly increased in the 1355A anthers at developmental stage 8. Furthermore, immunofluorescence studies using antibodies to the de‐esterified and esterified homogalacturonan (JIM5 and JIM7) showed that the Ghnsp mutant exhibits abundant of de‐esterified homogalacturonan in the tapetum and exine, coupled with defective exine formation. The characterization of GhNSP provides new understanding of the role of polygalacturonase and de‐esterified homogalacturonan in pollen exine formation.
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影响因子：
11.6
作者：
BROWN, SM;CROUCH, ML
通讯作者：
CROUCH, ML
影响因子：
7.4
作者：
Dobritsa, Anna A.;Lei, Zhentian;Sumner, Lloyd W.
通讯作者：
Sumner, Lloyd W.
影响因子：
27.5
作者：
Jia, Qi-Shi;Zhu, Jun;Yang, Zhong-Nan
通讯作者：
Yang, Zhong-Nan
DOI：
10.1016/j.ijbiomac.2014.07.010
发表时间：
2014-09-01
影响因子：
8.2
作者：
Kumar, Vineet;Nagar, Shipra;Tripathi, Y. C.
通讯作者：
Tripathi, Y. C.
影响因子：
7.2
作者：
Rhee, SY;Somerville, CR
通讯作者：
Somerville, CR