Prokaryotic successions and diversity in composts as revealed by 454-pyrosequencing
Prokaryotic successions and diversity in composts as revealed by 454-pyrosequencing
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DOI：
10.1016/j.biortech.2013.01.138
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发表时间：
2013-04-01
影响因子：
11.4
通讯作者：
Hickey, William J.
中科院分区：
文献类型：
--
作者：
de Gannes, Vidya;Eudoxie, Gaius;Hickey, William J.
在这项研究中，454-焦磷酸测序被应用于分析原核模式在三个木质纤维素堆肥系统的三个主要阶段。在所有堆肥，多样性扩大堆肥的进展。所有堆肥的中温阶段和成熟阶段的群落是不同的，这不支持存在于中温阶段的生物在高温期间进入休眠，并在成熟阶段重新定殖堆肥的概念。相似性分析表明，堆肥阶段是一个显着的差异性来源（p = 0.011），堆肥类型不是（p = 0.401）。方差分析还显示了对丰度的显著阶段效应（p值）：藻类（0.001）、浮菌（0.002）、绿球藻（0.016）、三角形变形菌（0.027）、细菌（0.046）和γ-变形菌（0.056）。成熟期堆肥是一个首选的生态位为的degreea，浮游菌，Chloroflexi和Deltaproteobacteria，而Gammaproteobacteria占主导地位的早期阶段。因此，成熟阶段的模式可能会影响堆肥成熟的生物标志物检测的发展。(C)2013爱思唯尔有限公司保留所有权利。
In this study, 454-pyrosequencing was applied to analyze prokaryotic patterns in three lignocellulosic composting systems across the three main phases. In all composts, diversity expanded as composting progressed. Communities in the mesophilic- and mature-phases of all composts were distinct, which did not support the concept that organisms present in the mesophilic phase enter dormancy during thermophilic period, and re-colonize the compost at the mature phase. Analysis of similarity revealed compost phase was a significant source of dissimilarity (p = 0.011), compost type was not (p = 0.401). Analysis of variance also showed significant phase effects on the abundance of (p-value): Archaea (0.001), Planctomycetes (0.002), Chloroflexi (0.016), Deltaproteobacteria (0.027), Bacteria (0.046) and Gammaproteobacteria (0.056). Mature-phase compost was a preferred niche for the Archaea, Planctomycetes, Chloroflexi and Deltaproteobacteria, while Gammaproteobacteria were predominant in earlier phases. Thus, the mature phase pattern could have implications in the development of biomarker assays for compost maturity. (C) 2013 Elsevier Ltd. All rights reserved.