Evidence for a quantitative tissue-specific distribution of high mobility group chromosomal proteins.
Evidence for a quantitative tissue-specific distribution of high mobility group chromosomal proteins.
复制标题
高迁移率组染色体蛋白定量组织特异性分布的证据。
DOI：
10.1021/bi00560a003
复制
发表时间：
1980
期刊：
影响因子：
2.9
通讯作者：
Williams,DL
中科院分区：
文献类型：
--
作者：
Gordon,JS;Rosenfeld,BI;Kaufman,R;Williams,DL
Joel S. Gordon，* 巴里I. Rosenfeld，Randall考夫曼，和大卫L.威廉姆斯 * 摘要：研究了高迁移率族（HMG）染色体蛋白的定量组织特异性。四种不同鸡组织和红细胞的高氯酸（PCA）提取物中含有三种蛋白质，它们在NaDodS 04-聚丙烯酰胺凝胶上与红细胞核中的HMG 1，2和E共迁移。这三种来自胚胎骨骼肌和红细胞的HMG也在二维凝胶上共迁移，在第一维中采用酸-尿素系统，在第二维中采用NaDodS 〇 4系统。二维凝胶的解释表明，这三个低分子量的蛋白质产生的HMG 2带的酸-尿素凝胶。这些已被指定为HMG 2A和HMG 2B。在小牛胸腺PCA提取物中还发现了三种分子量相似的蛋白质。他们被安排在一个相似但不完全相同的
Joel S. Gordon,* Barry I. Rosenfeld, Randall Kaufman, and David L. Williams* abstract: The quantitative tissue specificity of the high mobility group (HMG) chromosomal proteins was investi-gated. Perchloric acid (PCA) extracts of four different chicken tissues and erythrocytes contained three proteins which co-migrated on NaDodS04-polyacrylamide gels with the HMG’s 1, 2, and E from erythrocyte nuclei. These three HMG’s from embryonic skeletal muscle and erythrocytes also comigrated on two-dimensional gels, employing an acid-urea system in the first dimension and an NaDodS04 system in the second. Interpretation of the two-dimensional gels suggested that the two low molecular weight proteins of this triplet arose from the HMG 2 band of the acid-urea gels. These have been designated HMG 2A and HMG 2B. Three proteins of similar molecular weights were also found in the PCA extracts of calf thymus. They were arranged in a similar but not identical