Green fluorescent protein-based biosensor for detecting SOS-inducing activity of genotoxic compounds
Green fluorescent protein-based biosensor for detecting SOS-inducing activity of genotoxic compounds
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DOI：
10.1016/s0167-7012(01)00335-9
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发表时间：
2002-01-01
影响因子：
2.2
通讯作者：
Trevors, JT
中科院分区：
文献类型：
--
作者：
Kostrzynska, M;Leung, KT;Trevors, JT
日益严重的环境污染需要采用特定且灵敏的方法来检测水、食品和环境样品中的遗传毒性物质。遗传毒性评估测试通常基于对化学物质引起的 DNA 损伤做出反应的生物传感器菌株。在本研究中，已开发出荧光报告基因大肠杆菌菌株，其包含DNA损伤诱导型recA启动子和绿色荧光蛋白基因（gfp）或编码红移、更高强度GFP变体（突变体3）的基因之间的质粒转录融合。基于 GFP 的生物传感器可以检测对丝裂霉素 C (MMC)、N-甲基-N'-硝基-,V-亚硝基胍 (MNNG) 和萘啶酸 (NA) 等基因毒性剂的剂量依赖性反应。携带recA'-gfp突变体3融合体的报告菌株比含有野生型gfp的菌株产生更显着和更灵敏的反应。这些结果表明基于recA'-gfp突变体3的生物传感器对于基因毒素的检测具有潜在的用途。 (C) 2002 年，Elsevier Science B.V. 出版
Increasing levels of environmental pollution demand specific and sensitive methods for detection of genotoxic agents in water, food products and environmental samples. Tests for genotoxicity assessment are often based on biosensor strains that respond to DNA damage induced by chemicals. In the present study, fluorescent reporter Escherichia coli strains have been developed, which contain a plasmid-borne transcriptional fusion between the DNA-damage inducible recA promoter and the green fluorescent protein gene (gfp) or a gene encoding a red-shifted, higher intensity GFP variant (mutant 3). GFP-based biosensors allowed the detection of a dose-dependent response to genotoxic agents such as mitomycin C (MMC), N-methyl-N'-nitro-,V-nitrosoguanidine (MNNG) and nalidixic acid (NA). A reporter strain carrying recA'-gfp mutant 3 fusion gave more dramatic and sensitive response than a strain containing the wild-type gfp. These results indicate that recA'-gfp mutant 3-based biosensor is potentially useful for detection of genotoxins. (C) 2002 Published by Elsevier Science B.V.