A molecular analysis of dietary diversity for three archaic Native Americans
A molecular analysis of dietary diversity for three archaic Native Americans
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DOI：
10.1073/pnas.061014798
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发表时间：
2001-04-10
影响因子：
11.1
通讯作者：
Pääbo, S
中科院分区：
文献类型：
--
作者：
Poinar, HN;Kuch, M;Pääbo, S
DNA是从三个粪便样本中提取的，这些样本来自德克萨斯州的Hinds Cave，有2,000多年的历史。人类mtDNA序列的扩增显示他们与当代美洲原住民的联系，而叉角羚羊、大角羊和棉尾兔的序列则使这些动物被确定为这些个体饮食的一部分。此外，叶绿体DNA序列的扩增确定了8种不同的植物作为膳食元素。这些古人类在很短的时间内食用了2-4种不同的动物物种和4-8种不同的植物物种，从古粪便中提取DNA的成功率与从骨骼遗骸中提取DNA的成功率形成强烈对比，骨骼遗骸的成功率通常很低。因此，人类古粪便遗骸代表了古代DNA的来源，其显著补充并且在某些情况下可能上级于来自骨骼组织的DNA。
DNA was extracted from three fecal samples, more than 2,000 years old, from Hinds Cave, Texas. Amplification of human mtDNA sequences showed their affiliation with contemporary Native Americans, while sequences from pronghorn antelope, bighorn sheep, and cottontail rabbit allowed these animals to be identified as part of the diet of these individuals. Furthermore, amplification of chloroplast DNA sequences identified eight different plants as dietary elements. These archaic humans consumed 2-4 different animal species and 4-8 different plant species during a short time period, The success rate for retrieval of DNA from paleofeces is in strong contrast to that from skeletal remains where the success rate is generally low. Thus, human paleofecal remains represent a source of ancient DNA that significantly complements and may in some cases be superior to that from skeletal tissue.