Effect of C-Type Natriuretic Peptide on Maturation and Developmental Competence of Goat Oocytes Matured In Vitro.
Effect of C-Type Natriuretic Peptide on Maturation and Developmental Competence of Goat Oocytes Matured In Vitro.
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DOI：
10.1371/journal.pone.0132318
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Ma B
中科院分区：
文献类型：
--
作者：
Zhang J;Wei Q;Cai J;Zhao X;Ma B
卵母细胞在体外成熟（IVM）过程中，由于核和胞质成熟的不同步，其发育能力受到损害。为了提高IVM效率，已经建立了成熟前培养或两步成熟策略，包括由药理学试剂诱导的减数分裂阻滞，以提供卵母细胞足够的时间来同步核和细胞质的成熟。C型利钠肽（CNP）作为一种卵母细胞成熟抑制剂，为提高体外成熟卵母细胞的发育能力提供了新的途径。然而，CNP对山羊卵母细胞减数分裂阻滞和成熟的影响尚不清楚。在本研究中，CNP显示在山羊卵母细胞中起OMI的作用。CNP可以暂时维持山羊卵母细胞体外培养4小时的减数分裂停滞。这种短暂的效应部分是由于利钠肽受体2（Npr2）的减少。Escherichia coli能延缓Npr2表达的下降，延长减数分裂停滞时间达6小时。在此基础上，建立了山羊卵母细胞成熟的两步法，可显著提高卵母细胞的成熟率。孤雌激活后，卵裂率、囊胚率和囊胚细胞总数均显著提高。结果表明，CNP可以延缓山羊卵母细胞减数分裂的恢复，提高其体外发育能力。
The developmental competence of oocytes during in vitro maturation (IVM) is compromised due to asynchronous nuclear and cytoplasmic maturation. To improve IVM efficiency, a pre-maturation culture or two-step maturation strategy has been established, involving meiosis arrest induced by pharmacological agents to provide oocytes with sufficient time to synchronize the maturation of the nucleus and cytoplasm. C-type natriuretic peptide (CNP), which has been demonstrated to function as an oocyte maturation inhibitor (OMI) in many species, provides a new alternative to improve the developmental capacity of oocytes matured in vitro. However, the effect of CNP on meiosis arrest and the maturation of goat oocytes remains unclear. In the present study, CNP was shown to function as an OMI in goat oocytes. CNP could temporarily maintain the meiotic arrest of goat oocytes cultured in vitro for 4 hours. This transient effect was partly due to the reduction of natriuretic peptide receptor 2 (Npr2). Estradiol could delay the decrease in Npr2 expression and prolong the duration of meiosis arrest up to 6 hours. Based on the above results, a two-step method was established for goat oocyte maturation, in which the oocyte maturation rate was significantly increased. After parthenogenetic activation, the cleavage rate, blastocyst rate and total cell number of blastocysts were significantly improved. Our results suggested that CNP can be used to delay meiotic resumption and enhance the developmental competence of goat oocytes matured in vitro.