Developmental and photoregulation of three Neurospora crassa carotenogenic genes during conidiation induced by desiccation
Developmental and photoregulation of three Neurospora crassa carotenogenic genes during conidiation induced by desiccation
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DOI：
10.1006/fgbi.1996.0948
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发表时间：
1997-02-01
影响因子：
3
通讯作者：
Schmidhauser, TJ
中科院分区：
文献类型：
--
作者：
Li, CG;Sachs, MS;Schmidhauser, TJ
粗糙脉孢菌无性孢子形成（分生孢子形成）由不同的线索诱导，包括干燥的气生环境。在该发育途径期间表达的三种基因是编码类胡萝卜素生物合成酶的白化病（al）基因。如果分生孢子形成由液体培养物中的营养剥夺诱导，则表达两种重叠的al-3 mRNA，al-3（m）和al-3（c）（Arpaia等人，1995，Dev，Biol，170，620-635）。在此，我们定量了四种白化病基因信使al-1、al-2、al-3（m）和al-3（c）中每一种在分生孢子野生型培养物中和在光诱导的胡萝卜素生成缺陷的两种突变体wc-1和wc-2的培养物中的积累。在四种白化病基因转录物中，在黑暗中分生孢子的野生型培养物中，只有α 1 -1的表达受到发育调节。所有四种白化病基因转录本的表达在分生孢子的wc-1或wc-2培养物中受到发育调节。与其它白化病基因转录本不同，α 1 -3（c）的表达仅在分生孢子形成早期受到光调节。在干燥的fld和fl培养物中，al-1、al-2和al-3（m）转录物积累观察到早期达到峰值然后下降，而al-3（c）转录物积累随着时间增加。(C)北京：科学出版社.
Neurospora crassa asexual sporulation (conidiation) is induced by different cues including desiccating aerial environments, Three of the genes that are expressed during this developmental pathway are the albino (al) genes, which encode carotenoid biosynthetic enzymes, If conidiation is induced by nutrient deprivation in liquid culture, two overlapping al-3 mRNAs, al-3 (m) and al-3 (c), are expressed (Arpaia et al., 1995, Dev, Biol, 170, 620-635), Here we quantitate accumulation of each of the four albino gene messages, al-1, al-2, al-3 (m), and al-3 (c), in conidiating wild-type cultures and in cultures of two mutants defective in photoinduced carotenogenesis, wc-1 and wc-2, Of the four albino gene transcripts, only al-1 expression is developmentally regulated in wild-type cultures conidiating in darkness, Expression of all four albino gene transcripts is developmentally regulated in conidiating wc-1 or wc-2 cultures, Unlike other albino gene transcripts, expression of al-3 (c) is photoregulated only early in conidiation. The fld and fl mutants are defective in conidiation with development halting at distinct early stages of spore formation, In desiccated fld and fl cultures al-1, al-2, and al-3 (m) transcript accumulation is observed to peak early and then decline, while al-3 (c) transcript accumulation increases with time. (C) 1997 Academic Press.