Effects of lipopolysaccharide on newly established rat dental pulp-derived cell line with odontoblastic properties
Effects of lipopolysaccharide on newly established rat dental pulp-derived cell line with odontoblastic properties
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DOI：
10.1016/j.joen.2007.05.015
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发表时间：
2007-10-01
影响因子：
4.2
通讯作者：
Nishihara, Tatsuji
中科院分区：
文献类型：
--
作者：
Nomiyama, Kimiko;Kitamura, Chiaki;Nishihara, Tatsuji
为了阐明牙髓创伤愈合和再生的机制，建立连续的成牙本质细胞系是非常重要的。本研究以大鼠切牙为材料，建立了牙髓前体细胞系。这些细胞系表现出高水平的碱性磷酸酶（ALP）活性，Runx2和牙本质涎磷蛋白（DSPP）的表达，和矿化结节的细胞外形成。通过使用具有高表达水平的DSPP和显著的矿物质沉积的细胞系，我们研究了细菌脂多糖（LPS）是否对其成牙本质细胞特性具有影响，发现ALP活性、DSPP和Runx 2的表达以及矿化结节的形成以LPS剂量依赖性方式受到抑制。这些结果表明，我们建立的牙髓祖细胞系表现出成牙本质细胞的特性，这是由LIPS抑制，这表明革兰氏阴性细菌感染可能下调成牙本质细胞的功能。
To clarify mechanisms of pulp wound healing and regeneration, it is important to establish continuous odontoblast-lineage cell lines. In this study, we established the proliferating pulp progenitor cell lines from dental papilla cells of rat incisor. These cell lines showed high levels of alkaline phosphatase (ALP) activity, expression of Runx2 and dentin sialophosphoprotein (DSPP), and extracellular formation of mineralized nodules. By using the cell line with high expression level of DSPP and the prominent mineral deposition, we examined whether bacterial lipopolysaccharide (LPS) had effects on its odontoblastic properties and found that ALP activity, expression of DSPP and Runx2, and the formation of mineralized nodules were suppressed in LPS dose-dependent manner. These results indicate that our established pulp progenitor cell line exhibits odontoblastic properties, which were suppressed by LIPS, suggesting that gram-negative bacterial infection might downregulate the odontoblast function.