Genetic control of susceptibility to Cryptococcus neoformans in mice.
Genetic control of susceptibility to Cryptococcus neoformans in mice.
复制标题
小鼠对新型隐球菌易感性的遗传控制。
DOI：
10.1128/iai.29.2.494-499.1980
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发表时间：
1980
影响因子：
3.1
通讯作者：
Urba,WJ
中科院分区：
文献类型：
--
作者：
Rhodes,JC;Wicker,LS;Urba,WJ
Inbred mice injected intravenously with 5 × 106cells ofCryptococcus neoformansshowed two patterns of survival: sensitive (A/WySn, A.BY, A/J, DBA/2J, NZB/B1NJ, and SWR/J) and resistant [C57BL/10Sn, B10.A, B10.A (2R), B10.S (7R),C57BR/cdJ, C58/J, C3H/HeJ, BALB/c, DBA/1J, and SJL/J]. Relative susceptibility based on survival time was shown to correspond to differences obtained for 50% lethal dose values. Either decreasing the dose of organisms or changing to the intraperitoneal route of inoculation resulted in prolonged survival times, but neither change affected the observed patterns of survival. F1 hybrids between different sensitive strains were also sensitive, whereas F1 hybrids between sensitive and resistant strains were resistant, indicating a dominant mode of inheritance. Sensitivity and resistance were shown to be under single gene control by segregation analysis in F2 progeny produced by inbreeding (B10.A × A/WySn)F1 hybrids and in (F1 × A/WySn) backcross progeny. Blood obtained from parental strains, F1, F2, and backcross hybrids was tested for the presence or absence of hemolytic complement. Mice lacking hemolytic complement activity in their sera are homozygous for theHc0allele at theHclocus on chromosome 2 and are deficient in the complement component C5. A 1:1 correspondence was found between C5 deficiency and sensitivity toC. neoformans.Resistance was shown to cosegregate with the presence of hemolytic complement in the F2 and the backcross progenies.
