Controlled release, antimicrobial activity, and oral mucosa irritation of cetylpyridinium chloride-montmorillonite incorporated in a tissue conditioner
Controlled release, antimicrobial activity, and oral mucosa irritation of cetylpyridinium chloride-montmorillonite incorporated in a tissue conditioner
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DOI：
10.4012/dmj.2021-155
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发表时间：
2022-02-01
影响因子：
2.5
通讯作者：
Yokoyama, Atsuro
中科院分区：
文献类型：
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作者：
Asahara, Erika;Abe, Yasuhiko;Yokoyama, Atsuro
本研究考察了含氯化十六烷基吡啶-蒙脱土（CPC- mont）的组织调节剂（TC）对氯化十六烷基吡啶（CPC）的控释、抗菌活性和对口腔黏膜的刺激性。在蒸馏水、0.2 M NaCl和0.2 M HCl三种测试溶液中进行CPC释放试验，每天进行28 d。按照JIS Z 2801/ISO 22196标准，测定其对白色念珠菌、金黄色葡萄球菌和变形链球菌7、14、21和28天的抑菌活性。根据ISO 1099310:2010标准，用5只雄性仓鼠的颊袋进行口腔黏膜刺激试验。每日CPC释放量和28天累积释放量（6.12 mg）均小于咽喉痛药的每日安全最大值（8 mg）。此外，含有CPC-Mont的TC对黏附菌的抗菌活性可维持14天，且对口腔黏膜无刺激作用。
This study examined the controlled release of cetylpyridinium chloride (CPC) from a tissue conditioner (TC) containing CPC-montmorillonite (CPC-Mont), the associated antimicrobial activity, and oral mucosa irritation. The CPC release test was performed daily for 28 days in three test solutions: distilled water, 0.2 M NaCl, and 0.2 M HCl. The antimicrobial activities for 7, 14, 21, and 28 days against Candida albicans, Staphylococcus aureus, and Streptococcus mutans were assessed according to the JIS Z 2801/ISO 22196 standard. An oral mucosa irritation test was conducted using cheek pouches in five male hamsters according to the ISO 1099310:2010 standard. The amount of CPC released each day and the cumulative amount released over 28 days (6.12 mg) were less than the daily safe maximum of sore throat medicines (8 mg). Additionally, TC with CPC-Mont could sustain antimicrobial activity against adherent bacteria for 14 days and has no oral mucosa irritation potential.