Venomics and antivenomics profiles of North African Cerastes cerastes and C. vipera populations reveals a potentially important therapeutic weakness
Venomics and antivenomics profiles of North African Cerastes cerastes and C. vipera populations reveals a potentially important therapeutic weakness
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DOI：
10.1016/j.jprot.2012.02.021
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发表时间：
2012-04-18
影响因子：
3.3
通讯作者：
Calvete, Juan J.
中科院分区：
文献类型：
--
作者：
Fahmi, Laila;Makran, Bouchra;Calvete, Juan J.
我们报告了来自摩洛哥的医学相关蛇Cerastes Cerastes毒液的蛋白质组学分析，以及实验单特异性（针对摩洛哥C. Cerastes毒液的CcMo_AV）和商业（针对突尼斯C. Cerastes和M. lebetina毒液的Gamma-VIP） F(ab')(2)抗蛇毒血清对C. Cerastes和C. vipera毒液的地理变异的免疫反应性谱。C. cerastes毒液是一个低复杂度的蛋白质组，由6个蛋白家族的25-30种毒素组成，主要作用于止血系统。这种毒素库解释了在C. cereces中毒中观察到的临床图像。尽管地理成分存在差异，但突尼斯巴斯德研究所生产的单特异性CcMo_AV和γ - vip二价抗蛇毒血清对摩洛哥、突尼斯和埃及C. ceraastes毒液蛋白具有相似的免疫捕获能力。部分逃避免疫识别的蛋白均被鉴定为PLA(2)分子。抗蛇毒分析显示，摩洛哥CcMo_AV和突尼斯Gamma-VIP抗蛇毒血清对C. vipera毒液的交叉反应程度较低。本研究表明，在毒液免疫混合物的配方中加入蝮蛇毒液可以实现更完整的治疗覆盖，从而产生泛cereces抗蛇毒血清。(C) 2012 Elsevier B.V.版权所有
We report the proteomic analysis of the venom of the medically relevant snake, Cerastes cerastes, from Morocco, and the immunoreactivity profile of an experimental monospecific (CcMo_AV against Moroccan C. cerastes venom) and a commercial (Gamma-VIP against Tunisian C. cerastes and M. lebetina venoms) F(ab')(2) antivenoms towards geographic variants of C. cerastes and C. vipera venoms. The venom of C. cerastes is a low-complexity proteome composed of 25-30 toxins belonging to 6 protein families, mainly targetting the hemostatic system. This toxin arsenal explains the clinical picture observed in C. cerastes envenomings. Despite geographic compositional variation, the monospecific CcMo_AV and the Gamma-VIP divalent antivenom produced at Institut Pasteur de Tunis, showed similar immunocapturing capability towards Moroccan, Tunisian, and Egyptian C. cerastes venom proteins. Proteins partially escaping immunorecognition were all identified as PLA(2) molecules. Antivenomic analysis showed low degree of cross-reactivity of Moroccan CcMo_AV and Tunisian Gamma-VIP antivenoms towards C. vipera venom toxins. This study indicates that a more complete therapeutic cover could be achieved by including C. vipera venom in the formulation of venom immunization mixtures, thereby generating a pan-Cerastes antivenom. (C) 2012 Elsevier B.V. All rights reserved.