Comparison of base inclination of ribo-GC and deoxyribo-GC polymers, and synthesis of poly(rGrC)-poly(rGrC).
Comparison of base inclination of ribo-GC and deoxyribo-GC polymers, and synthesis of poly(rGrC)-poly(rGrC).
复制标题
核糖-GC和脱氧核糖-GC聚合物的碱基倾斜度比较，以及聚(rGrC)-聚(rGrC)的合成。
DOI：
10.1002/bip.360360306
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
JohnsonJr,WC
中科院分区：
文献类型：
--
作者：
Jin,X;JohnsonJr,WC
通过使用流动线性二色性（LD）测量缓冲液中ribo-GC聚合物的基线法线与螺旋轴之间的倾斜角以及基线倾斜所围绕的轴，并与缓冲液中和脱水条件下脱氧ribo-GC聚合物的测量结果进行比较。设计了一种新的方法来大量合成聚（rGrC）-聚（rGrC），这是商业上不可用的。该RNA的LD显示出与缓冲液中的B-型DNA相似的倾斜角，尽管轴不同。在脱水条件下，poly（dGdC）-poly（dGdC）的CD与poly（rGrC）-poly（rGrC）相似，表明其为A型，并且LD给出比B型或相应RNA更大的倾斜角。Poly（dG）-Poly（dC）在缓冲液中为A型。缓冲液中的poly（rG）-poly（rC）和缓冲液中的poly（dG）-poly（dC）以及脱水条件下的poly（dG）-poly（dC）之间的比较揭示了相似的倾斜角和轴，尽管LD显示DNA具有最大的倾斜角。除了具有独特的二向色性降低的poly（rGrC）-poly（rGrC）之外，G的所有轴都相似，C的轴也是如此。John Wiley & Sons，Inc.
The inclination angle between the base normal and the helix axis, and the axes around which the bases incline, are measured for ribo‐GC polymers in buffer by using flow linear dichroism (LD), and compared to measurements for deoxyribo‐GC polymers in buffer and under dehydrating conditions. A new method is designed to synthesize poly(rGrC) ‐poly(rGrC), which is not available commercially, in large quantities. The LD of this RNA reveals inclination angles that are similar to the B‐form DNA in buffer, although the axes are different. The CD of poly(dGdC)‐poly(dGdC) under the dehydrating conditions is similar to poly(rGrC)‐poly(rGrC), indicating it is in the A form, and the LD gives larger inclination angles than either the B form or the corresponding RNA. Poly(dG)‐poly(dC) is in the A form in buffer. Comparison among poly(rG)‐poly(rC) in buffer, and poly (dG)‐poly(dC) in buffer and under dehydrating conditions, reveals similar inclination angles and axes, although the LD shows that the DNA has the largest inclination angles. Except for poly(rGrC)‐poly(rGrC), which has a unique reduced dichroism, all the axes for G are similar, as are the axes for C. © 1995 John Wiley & Sons, Inc.