Mucin deficiency causes functional and structural changes of the ocular surface.
Mucin deficiency causes functional and structural changes of the ocular surface.
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DOI：
10.1371/journal.pone.0050704
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Chen Y
中科院分区：
文献类型：
--
作者：
Floyd AM;Zhou X;Evans C;Rompala OJ;Zhu L;Wang M;Chen Y
MUC5AC是眼系统中最丰富的凝胶形成粘蛋白。但具体功能不明。在本研究中，Muc5ac敲除（KO）小鼠模型与其野生型（WT）对照相比经受各种生理测量。有趣的是，当KO小鼠与WT小鼠相比时，平均泪液破裂时间（TBUT）值显著较低，角膜荧光素染色评分显著较高。但泪液量没有改变。尽管在KO小鼠的结膜中缺乏Muc5ac表达，但在这些小鼠中Muc5b表达显著增加。角膜混浊，不同的位置和严重程度，被发现在一些KO小鼠，但不是在WT小鼠。目前的结果表明，与WT小鼠相比，KO小鼠泪液的质量而非数量存在显著差异。干眼症是多因素的，因此进一步评估这些KO小鼠的泪膜、泪腺单位和角膜结构的不同组分可能有助于阐明粘蛋白在干眼症中的作用。由于Muc5ac基因敲除小鼠具有干眼的临床特征，因此该小鼠模型对于进一步研究人类干眼中眼表的病理生理学非常有用。
MUC5AC is the most abundant gel-forming mucin in the ocular system. However, the specific function is unknown. In the present study, a Muc5ac knockout (KO) mouse model was subject to various physiological measurements as compared to its wide-type (WT) control. Interestingly, when KO mice were compared to WT mice, the mean tear break up time (TBUT) values were significantly lower and corneal fluorescein staining scores were significantly higher. But the tear volume was not changed. Despite the lack of Muc5ac expression in the conjunctiva of KO mice, Muc5b expression was significantly increased in these mice. Corneal opacification, varying in location and severity, was found in a few KO mice but not in WT mice. The present results suggest a significant difference in the quality, but not the quantity, of tear fluid in the KO mice compared to WT mice. Dry eye disease is multifactorial and therefore further evaluation of the varying components of the tear film, lacrimal unit and corneal structure of these KO mice may help elucidate the role of mucins in dry eye disease. Because Muc5ac knockout mice have clinical features of dry eye, this mouse model will be extremely useful for further studies regarding the pathophysiology of the ocular surface in dry eye in humans.
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影响因子：
4.8
作者：
Katsumi, A;Tuley, EA;Sadler, JE
通讯作者：
Sadler, JE
影响因子：
--
作者：
Peral, Assumpta;Dominguez-Godinez, Carmen O.;Pintor, Jesus
通讯作者：
Pintor, Jesus
影响因子：
4.4
作者：
Pauly, Aude;Brignole-Baudouin, Francoise;Baudouin, Christophe
通讯作者：
Baudouin, Christophe
影响因子：
3.4
作者：
Spurr-Michaud, Sandra;Argueso, Pablo;Gipson, Ilene
通讯作者：
Gipson, Ilene
DOI：
10.1165/rcmb.2011-0340oc
发表时间：
2012-05-01
影响因子：
6.4
作者：
Tao, Shasha;Zhu, Lingxiang;Chen, Yin
通讯作者：
Chen, Yin