Bioprospecting of tropical microalgae for high-value products: n-3 polyunsaturated fatty acids and carotenoids
Bioprospecting of tropical microalgae for high-value products: n-3 polyunsaturated fatty acids and carotenoids
复制标题
DOI：
10.1016/j.aqrep.2022.101406
复制
发表时间：
2022-12-01
影响因子：
3.7
通讯作者：
Jusoh, Malinna
中科院分区：
文献类型：
--
作者：
Katayama, Tomoyo;Rahman, Norazira Abdu;Jusoh, Malinna
微藻是类胡萝卜素和多不饱和脂肪酸（PUFAs）的重要再生来源，具有很强的抗氧化、抗炎和保护肝脏的特性。在这项研究中，从马来西亚半岛的淡水、咸淡水和海水中分离、纯化和培养了本地微藻菌株。采用尼罗红荧光染料筛选试验检测微藻的脂质和类胡萝卜素含量，研究了微藻作为脂质和类胡萝卜素天然来源的潜力。在125株微藻中，从海水和咸淡水中分离的9株微藻（毛囊毛藻、小球藻、小球藻、寻常藻、黄衣毛藻、小头Nitzschia capitellata、异乳卵囊藻、滨海卵囊藻和weissflogii）显示出高荧光强度。然后，对这9株菌株进行生化组成分析。weissflogii海assiassira TRG10-p105菌株的n-3 PUFAs和类胡萝卜素产量最高。该菌株产生高水平的n-3 PUFAs，特别是二十碳五烯酸（EPA）。该菌株的EPA含量相对于干重（DW）和总脂肪酸含量（分别为10.8%和21.4%）显著高于目前在水产养殖等工业应用中报道的品种。海assiassira weissflogii TRG10-p105的总类胡萝卜素含量最高（20.2 mg g(-1) DW），主要是由于其岩藻黄质含量高（11.5 mg g(-1) DW）。本研究结果表明，T. weissflogii TRG10-p105可以作为热带地区的高价值微藻菌株，大量产生n-3 PUFAs和类胡萝卜素。由于该菌株对铵的耐受性较好，因此可在废弃物产生的富铵水中进行大规模生产，以降低藻类生产成本。
Microalgae are an important renewable source of carotenoids and polyunsaturated fatty acids (PUFAs), with strong antioxidant, anti-inflammatory, and hepatoprotective properties. In this study, indigenous microalgal strains from fresh, brackish, and marine waters of Peninsular Malaysia were isolated, purified, and cultured. The potential of microalgae as a natural source of lipids and carotenoids was studied using a screening test with Nile red fluorescence dye to examine the lipid and carotenoid contents. Of 125 microalgae strains, nine strains isolated from marine and brackish water samples (Chaetoceros gracilis, Chlorella sp., C. sorokiniana, C. vulgaris, Tisochrysis lutea, Nitzschia capitellata, Oocystella heteromucosa, Oocystis marina, and Thalassiosira weissflogii) showed high fluorescence intensities. Next, the biochemical composition of these nine strains was examined. Thalassiosira weissflogii (strain TRG10-p105) showed the highest production of n-3 PUFAs and carotenoids among the nine strains. This strain produced high levels of n-3 PUFAs, particularly eicosapentaenoic acid (EPA). In this strain, the EPA contents relative to the dry weight (DW) and total fatty acid content (10.8% and 21.4%, respectively) were considerably higher than those reported in species currently used in industrial applications, such as aquaculture. Thalassiosira weissflogii TRG10-p105 had the highest total carotenoid content (20.2 mg g(-1) DW), primarily because of its high fucoxanthin content (11.5 mg g(-1) DW). The findings of this study indicate that T. weissflogii TRG10-p105 can serve as a high-value microalgal strain from tropical regions that produces n-3 PUFAs and carotenoids in abundance. Because a high ammonium tolerance was observed in this strain, its large-scale production can be implemented in ammonium-rich water produced from waste products to reduce the cost of algae production.