Cytomorphometry of developing rat liver and its application to enzymic differentiation.
Cytomorphometry of developing rat liver and its application to enzymic differentiation.
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DOI：
10.1083/jcb.52.2.261
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发表时间：
1972-02
期刊：
影响因子：
--
通讯作者：
Knox WE
中科院分区：
文献类型：
--
作者：
Greengard O;Federman M;Knox WE
定量体视学方法已适用于测量肝实质细胞、造血细胞和枯否细胞的体积，以及测量这些细胞类型的相对和绝对数量（每单位体积）和实质细胞的平均体积。这些形态学参数是解释肝脏生化分化的主要参数。这些参数的定量变化，在大鼠肝脏之间的第15天的妊娠和成年生活，提出。尽管有大量的造血细胞，实质细胞填充超过一半的肝脏体积之间的第15和第18天的妊娠和0.85的肝脏体积在术语。库普弗细胞所占肝脏体积的比例从来不超过0.02;每立方厘米库普弗细胞的数量在胎儿和成人之间增加不到两倍。单个实质细胞的平均体积在胎儿晚期增加三倍，在新生儿期下降，在出生后第12天和第28天之间增加一倍。获得形态测定数据后，不可能将酶浓度（单位/克，在全肝匀浆中测定）转换为每单位体积实质组织或造血组织或每种类型的单个细胞的酶量。在晚期胎肝中，只有酶浓度升高不到两倍，这可能是由于以造血成分为代价的实质组织的富集。晚期胎儿肝簇（以及新生儿和晚期哺乳肝簇）的肝酶突然升高两倍以上，反映了每个实质质量和每个实质细胞的升高。甲状腺素和胰高血糖素，其中胎鼠的管理，促进酶的分化在肝脏中，是没有明显的影响细胞学参数的研究。氢化可的松加速胎肝造血组织的退化。产前注射氢化可的松减少的酶可能集中在造血细胞中。
Quantitative stereological methods have been adapted for the measurement of the volume of liver attributable to parenchymal, hematopoietic, and Kupffer cells and for the measurement of the relative and absolute number (per unit volume) of these cell types and the mean volume of the parenchymal cell. These morphological parameters are the main ones for interpreting the biochemical differentiation of liver. Quantitative changes in these parameters, in rat liver between the 15th day of gestation and adult life, are presented. Despite the large number of hematopoietic cells, the parenchymal cells fill more than half of the liver volume between the 15th and 18th days of gestation and 0.85 of the liver volume at term. The fraction of liver volume occupied by Kupffer cells is never more than 0.02; the number of Kupffer cells per cubic centimeter increases less than twofold between fetal and adult life. The mean volume of individual parenchymal cells undergoes a threefold rise during late fetal life, declines in the neonatal period, and doubles between the 12th and 28th postnatal days. With the morphometric data obtained, it is impossible to convert enzyme concentrations (units per gram, determined in homogenates of whole liver) to enzyme amounts per unit volume of parenchymal or hematopoietic tissue or per individual cell of either type. In late fetal liver, only rises in enzyme concentration less than twofold may be attributed to the enrichment of parenchymal tissue at the expense of hematopoietic elements. The sudden upsurge, by more than twofold, of hepatic enzymes of the late fetal cluster (and also of the neonatal and late suckling cluster) reflects rises per parenchymal mass and per parenchymal cell. Thyroxine and glucagon, the administration of which to fetal rats promotes enzyme differentiation in liver, are without appreciable effect on the cytological parameters studied. Hydrocortisone accelerates the involution of hematopoietic tissue in fetal liver. Enzymes that are diminished by prenatal injection of hydrocortisone may be concentrated in hematopoietic cells.