Maltophilin: A new antifungal compound produced by Stenotrophomonas maltophilia R3089
Maltophilin: A new antifungal compound produced by Stenotrophomonas maltophilia R3089
复制标题
DOI：
10.7164/antibiotics.49.1101
复制
发表时间：
1996-11-01
影响因子：
3.3
通讯作者：
Bahl, H
中科院分区：
文献类型：
--
作者：
Jakobi, M;Winkelmann, G;Bahl, H
从油菜植物（甘蓝型油菜L.）的根际分离的嗜麦芽窄食单胞菌R3089菌株，产生了一种新的抗真菌化合物，命名为maltophilin，它抑制各种腐殖酸，人类病原性和植物病原性真菌的生长，但对革兰氏阳性和革兰氏阴性细菌无活性。嗜麦芽窄食单胞菌素是一种新型大环内酰胺类抗生素，分子量为510 μ。通过乙酸乙酯萃取和Sephadex LH 20凝胶过滤从培养滤液中分离化合物，并通过反相制备型HPLC纯化。通过电喷雾质谱和H-1和C-13 NMR光谱鉴定了Maltophilin的结构。
Strains of Stenotrophomonas maltophilia R3089, isolated from the rhizosphere of rape plants (Brassica napus L.), produced a novel antifungal compound, named maltophilin, which inhibited the growth of various saprophytic, human-pathogenic and phytopathogenic fungi but was inactive against Gram-positive and Gram-negative bacteria. Maltophilin is a novel macrocyclic lactam antibiotic with a molecular mass of 510 mu. The compound was isolated from the culture filtrate by ethyl acetate extraction and gel filtration on Sephadex LH20 and purified by preparative HPLC on reversed phase. The structure of maltophilin was elucidated by electrospray mass spectrometry and H-1 and C-13 NMR spectroscopy.