Detection of acid-sensing ion channel 3 (ASIC3) in periodontal Ruffini endings of mouse incisors
Detection of acid-sensing ion channel 3 (ASIC3) in periodontal Ruffini endings of mouse incisors
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DOI：
10.1016/j.neulet.2010.11.023
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发表时间：
2011-01-20
影响因子：
2.5
通讯作者：
Maeda, Takeyasu
中科院分区：
文献类型：
--
作者：
Rahman, Farhana;Harada, Fumiko;Maeda, Takeyasu
酸感应离子通道 3 (ASIC3) 是上皮钠通道/简并蛋白 (ENaC/DEG) 超家族的成员，据报道参与酸感应、机械感觉和伤害感受。然而，没有关于该分子在牙周膜中的精确定位和功能的信息，牙周膜含有丰富的源自三叉神经节的感觉神经。本研究通过免疫组织化学检测了小鼠门牙舌侧牙周膜中 ASIC3 的表达。此外，在蛋白质（免疫组织化学和定量分析）和 mRNA 水平（RT-PCR 技术和原位杂交组织化学）方面研究了 ASIC3 在支配牙周膜的三叉神经节中的表达。 ASIC3 的免疫组织化学能够证明小鼠门牙牙周 Ruffini 末端的树突特征。没有终止为伤害性游离神经末梢的细纤维表现出 ASIC3 免疫反应性。双重免疫荧光染色显示轴浆中有 ASIC3 免疫反应，但普通雪旺细胞（包括相关的末端雪旺细胞）中没有。对三叉神经节的观察显示不同大小的神经元表达 ASIC3 免疫反应；使用特定 cRNA 探针进行原位杂交组织化学证实，中小型神经元具有最强烈的免疫阳性反应。对三叉神经节神经元的定量分析表明，38.0%的ASIC3神经元可归类为介导力传导的中等大小神经元。这些发现表明 ASIC3 在牙周鲁菲尼末梢中充当机械感觉分子。 (C) 2010 Elsevier Ireland Ltd. 保留所有权利。
The acid-sensing ion channel 3 (ASIC3), a member of the epithelial sodium channel/degenerin (ENaC/DEG) superfamily, has been reported to participate in acid sensing, mechanosensation, and nociception. However, no information is available regarding the precise localization and function of this molecule in the periodontal ligament, which contains abundant sensory nerves originating from the trigeminal ganglion. The present study examined the expression of ASIC3 in the lingual periodontal ligament of mouse incisors by immunohistochemistry. Furthermore, the expression of ASIC3 in the trigeminal ganglion which innervates the periodontal ligament - was investigated at protein (immunohistochemistry and quantitative analysis) and mRNA levels (RT-PCR technique and in situ hybridization histochemistry). Immunohistochemistry for ASIC3 was able to demonstrate dendritic profiles of the periodontal Ruffini endings in the mouse incisors. No thin fibers terminating as nociceptive free nerve endings exhibited ASIC3 immunoreactivity. Double immunofluorescent staining revealed ASIC3 immunoreaction in the axoplasm but not in the ordinary Schwann cells - including the associated terminal Schwann cells. Observation of the trigeminal ganglia showed variously sized neurons expressing ASIC3 immunoreaction; the most intense immunopositivity was found in the small and medium-sized neurons, as confirmed by in situ hybridization histochemistry using a specific cRNA probe. Quantitative analysis on trigeminal ganglion neurons showed that 38.0% of ASIC3 neurons could be categorized as medium-sized neurons which mediate mechanotransduction. These findings suggest that ASIC3 functions as a molecule for mechanosensation in the periodontal Ruffini endings. (C) 2010 Elsevier Ireland Ltd. All rights reserved.