Priming and boosting immunity to respiratory syncytial virus by recombinant replication-defective vaccinia virus MVA.
Priming and boosting immunity to respiratory syncytial virus by recombinant replication-defective vaccinia virus MVA.
复制标题
通过重组复制缺陷型牛痘病毒 MVA 启动和增强对呼吸道合胞病毒的免疫力。
DOI：
10.1016/s0264-410x(99)00257-1
复制
发表时间：
1999
期刊：
影响因子：
5.5
通讯作者：
Moss,B
中科院分区：
文献类型：
--
作者：
Wyatt,LS;Whitehead,SS;Venanzi,KA;Murphy,BR;Moss,B
用表达呼吸道合胞病毒（RSV）F或G糖蛋白的高度减毒的MVA痘苗病毒株对小鼠进行鼻内和肌内免疫，诱导的RSV抗体滴度高于RSV感染所获得的抗体滴度，并极大地限制了RS攻击病毒在上呼吸道和下呼吸道中的复制。此外，表达RSV F和G的重组MVA是稳定的，并且与两种单一重组病毒的组合一样具有免疫原性。通过用重组MVA肌内免疫加强先前用减毒RSV鼻内感染诱导的针对RSV F和G的抗体水平。这些数据支持进一步开发重组MVA作为RSV疫苗。
Intranasal and intramuscular immunizations of mice with the highly attenuated MVA strain of vaccinia virus expressing the respiratory syncytial virus (RSV) F or G glycoprotein induced higher RSV antibody titers than those achieved by infection with RSV and greatly restricted the replication of RS challenge virus in both the upper and lower respiratory tracts. In addition, a recombinant MVA expressing both RSV F and G was stable and was as immunogenic as a combination of two single recombinant viruses. The levels of antibodies to RSV F and G, induced by previous intranasal infection with attenuated RSV, were boosted by intramuscular immunization with recombinant MVA. These data support further development of recombinant MVA as a RSV vaccine.