Hollow chitosan–silica nanospheres for doxorubicin delivery to cancer cells with enhanced antitumor effect in vivo
Hollow chitosan–silica nanospheres for doxorubicin delivery to cancer cells with enhanced antitumor effect in vivo
复制标题
DOI：
10.1039/c0jm03234d
复制
发表时间：
2011-02
影响因子：
--
通讯作者：
Eryun Yan;Yilong Fu;Xue Wang;Yin Ding;Hanqing Qian;Chi‐Hwa Wang;Yong Hu;Xiqun Jiang
中科院分区：
文献类型：
--
作者：
Eryun Yan;Yilong Fu;Xue Wang;Yin Ding;Hanqing Qian;Chi‐Hwa Wang;Yong Hu;Xiqun Jiang
在这里，我们报道了以壳聚糖-聚丙烯酸（CS-PAA）纳米颗粒为模板合成杂化中空壳聚糖-二氧化硅纳米球（CS-二氧化硅纳米球）和基于CS-二氧化硅纳米粒子的阿霉素（DOX）递送。通过场发射扫描电子显微镜（FESEM）和X射线光电子能谱（XPS）对CS-Silica NPs的形貌和微观结构进行了表征。共焦激光扫描显微镜 (CLSM) 和流式细胞术实验表明，细胞对负载 DOX 的 CS-Silica NP 的摄取具有时间依赖性。此外，负载DOX的CS-Silica NPs的细胞内化和细胞内分布表明，DOX主要分布在细胞核中，而载体主要位于细胞质中。体内抗肿瘤反应表明，负载DOX的CS-二氧化硅杂化空心纳米球在体内表现出优于游离药物的抗肿瘤作用，这可能归因于增强的细胞摄取效率和药物有效递送至细胞核。
Here we report the synthesis of hybrid hollow chitosan–silica nanospheres (CS–Silica NPs) with chitosan–polyacrylic acid (CS–PAA) nanoparticles as the template and doxorubicin (DOX) delivery based on CS–Silica NPs. The morphology and the microstructure of CS–Silica NPs were characterized by field emission scanning electron microscopy (FESEM) and X-ray photoelectron spectroscopy (XPS). The confocal laser scanning microscopy (CLSM) and flow cytometry experiments showed that the cellular uptake of the DOX-loaded CS–Silica NPs was time dependent. In addition, cellular internalization and intracellular distribution of DOX-loaded CS–Silica NPs indicated that the DOX was mainly distributed in the cell nucleus while the carriers were primarily located in the cytoplasm. In vivo antitumor response indicated that the DOX loaded CS–Silica hybrid hollow nanospheres exhibited superior antitumor effect over the free drugin vivo, which might be ascribable to the enhanced cellular uptake efficiency and the effective delivery of drug to the cell nucleus.