One day of estradiol treatment enhances platelet aggregation at the site of microvascular injury without altering aggregation ex vivo.
One day of estradiol treatment enhances platelet aggregation at the site of microvascular injury without altering aggregation ex vivo.
复制标题
一天的雌二醇治疗可增强微血管损伤部位的血小板聚集，而不会改变离体聚集。
DOI：
10.1016/0024-3205(88)90675-3
复制
发表时间：
1988
期刊：
影响因子：
6.1
通讯作者：
Nelson,GH
中科院分区：
文献类型：
--
作者：
Rosenblum,WI;el-Sabban,F;Nelson,GH
小鼠皮下植入含0.5 mg雌二醇安慰剂的颗粒。在植入后第二天，通过用有害的光/染料刺激在体内损伤脑表面上的小动脉（软脑膜小动脉）的内皮，在脑表面上的小动脉中产生血小板聚集。伤害性刺激开始至血小板聚集出现的时间明显缩短（p<0.05）。01)在雌二醇处理的小鼠中。与这种聚集增强相反，当在富血小板血浆（PRP）中离体测试血小板时，在雌二醇处理的小鼠中，花生四烯酸钠或ADP的聚集没有改变。因此，在雌二醇处理的小鼠的损伤的软脑膜小动脉中观察到的增强的聚集可能不反映雌二醇对血小板本身的直接影响。相反，增强的聚集可能反映了雌二醇对内皮或邻近组织的影响。这种效果是在雌二醇达到妊娠水平后24小时内产生的。早期的研究表明，在高雌二醇水平下7-14天后，体内作用相似，因此，该作用的快速性令人感兴趣。快速效应表明对巨核细胞的作用不太可能解释该结果。
Mice were implanted subcutaneously with a pellet containing 0.5 mg estradiol placebo. On the day following implantation platelet aggregation was produced in arterioles on the brain surface (pial arterioles) by injuring their endothelium in vivo with a noxious light/dye stimulus. The time between the onset of the noxious stimulus and the appearance of platelet aggregates was significantly shortened (p<. 01) in the estradiol treated mice. In contrast to this enhancement of aggregation, when platelets were tested ex vivo in platelet rich plasma (PRP), aggregation to sodium arachidonate or to ADP was not altered in estradiol treated mice. Thus the enhanced aggregation observed in injured pial arterioles of estradiol treated mice may not reflect direct effects of estradiol on the platelet itself. Rather enhanced aggregation may reflect an effect of estradiol on endothelium or adjacent tissue. This effect was produced within 24 hours of achieving pregnancy levels of estradiol. The rapidity of the effect is of interest in light of earlier studies showing a similar in vivo action following 7–14 days of high estradiol levels. The rapid effect suggests that an action on megakaryocytes is an unlikely explanation for the results.