Estimation of the late postmortem interval using FTIR spectroscopy and chemometrics in human skeletal remains
Estimation of the late postmortem interval using FTIR spectroscopy and chemometrics in human skeletal remains
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使用 FTIR 光谱和化学计量学估计人类骨骼遗骸的死亡后期间隔
DOI：
10.1016/j.forsciint.2017.10.033
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发表时间：
2017-12-01
影响因子：
2.2
通讯作者：
Wang, Zhenyuan
中科院分区：
文献类型：
--
作者：
Wang, Qi;Zhang, Yinming;Wang, Zhenyuan
由于缺乏可靠准确的方法，确定人体骨骼遗骸的死后时间（PMI）是法医学中最重要和最具挑战性的任务之一。在本文中，我们研究了骨化学的变化，随着增加PMI在两个不同的实验条件下，使用傅里叶变换红外光谱（FTIR）结合化学计量学方法从56人尸体的配对骨样品被埋葬（放置在土壤中）和未埋葬（暴露在空气中）之间的时间间隔76和552天。主成分分析（PCA）的结果表明，这两种情况下的化学差异有显着的影响，遗体的分解速度。同时，采用遗传算法结合偏最小二乘法（GA-PLS）进行预测，埋骨和未埋骨的预测均方根误差（RMSEP）分别为50.93天和71.03天。此外，GA-PLS还发现蛋白质的酰胺I区和与脂肪酸相关的1390 cm（1）附近的区域具有规律性的变化，在PMI的估算中发挥了重要作用。这项研究说明了利用FTIR光谱和化学计量学作为一个有吸引力的替代估计PMI的人类遗骸和巨大的潜力，这些技术在真实的法医案件与自然条件下的可行性。(C)2017爱思唯尔B. V.保留所有权利。
Due to a lack of reliable and accurate methods, determining the postmortem interval (PMI) of human skeletal remains is one of the most important and challenging tasks in forensic medicine. In this paper, we studied the changes to bone chemistry with increasing PMI in two different experimental conditions using Fourier transform infrared (FTIR) spectroscopy in conjunction with chemometrics methods Paired bone samples collected from 56 human corpses were buried (placed in soil) and unburied (exposed to the air) for intervals between 76 and 552 days. The results of principle component analysis (PCA) showed the chemical differences of these two cases had a significant influence on the rate of decomposition of the remains. Meanwhile, satisfactory predictions were performed by the genetic algorithm combined with partial least-squares (GA-PLS) with the root mean square errors of prediction (RMSEP) of 50.93 days for buried bones and 71.03 days for unburied bones. Moreover, the amide I region of proteins and the area around 1390 cm (1), which is associated with fatty acids, were identified with regular changes by GA-PLS and played an important role in estimating PMI. This study illustrates the feasibility of utilizing FTIR spectroscopy and chemometrics as an attractive alternative for estimating PMI of human remains and the great potential of these techniques in real forensic cases with natural conditions. (C) 2017 Elsevier B.V. All rights reserved.