Biological control of blossom blight of alfalfa caused by Botrytis cinerea under environmentally controlled and field conditions
Biological control of blossom blight of alfalfa caused by Botrytis cinerea under environmentally controlled and field conditions
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DOI：
10.1094/pdis.2004.88.11.1246
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发表时间：
2004-11-01
期刊：
影响因子：
4.5
通讯作者：
Erickson, RS
中科院分区：
文献类型：
--
作者：
Li, GQ;Huang, HC;Erickson, RS
在苜蓿离体小花上测定了拮抗真菌和拮抗细菌对灰葡萄孢产孢的抑制作用。在离体条件下，研究了玫瑰粘帚霉、链状粘帚霉和深绿木霉对紫花苜蓿幼花和幼荚的保护作用。C.对B引起的荚腐病和种腐病进行了防治试验。在田间条件下的灰霉病。结果表明，供试的4种拮抗菌，C. rosea，G. catenulatum、Tatroviride和Trichothecium roseum对B的产孢有抑制作用。在分离的苜蓿小花上的灰霉病。两个C. rosea和G. catenulatum能有效抑制B对苜蓿豆荚的侵染。灰霉病菌在紫花苜蓿花期鲜花瓣上接种，其防效均大于深绿木霉。对B的显著抑制。cinerea，C. rosea和G.将小链孢菌接种到豆荚发育期的衰老花瓣上，观察到豆荚和种子上的小链孢菌。田间试验结果表明，C.紫花苜蓿植株上部施用玫瑰红显著抑制了B引起的角果腐烂和种子腐烂。灰葡萄，并显着增加苜蓿种子产量在每3年。这些研究表明，C. Rosea具有作为生物防治剂防治由B引起的苜蓿开花枯萎病的潜力。灰叶
Fungal and bacterial antagonists were tested for their inhibition of sporulation of Botrytis cinerea on detached alfalfa florets. Clonostachys rosea, Gliocladium catenulatum, and Trichoderma atroviride were evaluated for protecting young blossoms and pods of alfalfa from infection by R cinerea in vitro. C. rosea was further tested to control pod rot and seed rot caused by B. cinerea under field conditions. The results showed that four of the tested antagonists, C. rosea, G. catenulatum, T atroviride, and Trichothecium roseum, could inhibit sporulation by B. cinerea on detached alfalfa florets. Both C. rosea and G. catenulatum were effective in suppression of infection of alfalfa pods by B. cinerea when inoculated on fresh petals of alfalfa at the anthesis stage, and their efficacy was greater than that of Trichoderma atroviride. A significant suppression of B. cinerea by C. rosea and G. catenulatum on pods and seed of alfalfa was observed when they were inoculated on senescent petals at the pod-development stage. Results of a field trial indicated that C. rosea applied to upper parts of alfalfa plants significantly suppressed pod rot and seed rot caused by B. cinerea, and significantly increased seed production of alfalfa in each of 3 years. These studies show that C. rosea has potential as a biocontrol agent for control of alfalfa blossom blight caused by B. cinerea.