spoVIC, a new sporulation locus in Bacillus subtilis affecting spore coats, germination and the rate of sporulation.
spoVIC, a new sporulation locus in Bacillus subtilis affecting spore coats, germination and the rate of sporulation.
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spoVIC，枯草芽孢杆菌中的一个新的孢子形成位点，影响孢子衣、萌发和孢子形成率。
DOI：
10.1099/00221287-131-9-2409
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
J. Mandelstam
中科院分区：
文献类型：
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作者：
W. James;J. Mandelstam
枯草芽孢杆菌染色体上cysB附近的突变标志着一个新的孢子形成位点，spoVIC。它导致孢子在所有条件下比野生型孢子萌发更慢，并且从间接证据来看，它似乎不会改变对萌发L-丙氨酸的亲和力。突变体孢子的外壳蛋白（尤其是Mr = 12000的碱溶性外壳蛋白）有所缺失，孢子外壳层结构紊乱。突变株生长正常，孢子形成正常，直到阶段II，之后，其孢子形成延迟约2小时相比，野生型。这种延迟导致一些外壳蛋白的合成延长和其他蛋白的合成延迟。这可能是种子发芽不足的原因。携带spoVIC 610突变和gerE 36的双突变菌株缓慢地形成孢子。它的孢子具有非常少的外壳蛋白，对热、溶菌酶和有机溶剂敏感，但萌发和单独携带spoVIC突变的菌株一样。根据这些发现讨论了孢子衣在萌发中的作用。
A mutation near cysB on the Bacillus subtilis chromosome marks a new sporulation locus, spoVIC. It causes spores to germinate more slowly than those of the wild-type under all conditions and, from indirect evidence, it does not appear to alter the affinity for the germinant L-alanine. The mutant spores have some deficiency of coat proteins (particularly the alkalisoluble coat protein, Mr = 12 000) and the spore coat layers are disorganized. The mutant strain grows normally and sporulates normally until stage II, after which its sporulation is delayed by about 2 h compared to that of the wild-type. This delay results in the prolonged synthesis of some coat proteins and the late synthesis of others. The abnormal coat may be the cause of the germination deficiency. A double mutant strain carrying the spoVIC610 mutation together with gerE36 sporulates slowly. Its spores have very little coat protein, are sensitive to heat, lysozyme and organic solvents, but germinate as well as the strain carrying the spoVIC mutation alone. The role of the spore coat in germination is discussed in the light of these findings.