Neuropeptide Y-like immunoreactivity in rat cranial parasympathetic neurons: coexistence with vasoactive intestinal peptide and choline acetyltransferase.
Neuropeptide Y-like immunoreactivity in rat cranial parasympathetic neurons: coexistence with vasoactive intestinal peptide and choline acetyltransferase.
复制标题
大鼠颅副交感神经元中的神经肽 Y 样免疫反应性：与血管活性肠肽和胆碱乙酰转移酶共存。
DOI：
10.1073/pnas.84.10.3511
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发表时间：
1987
影响因子：
11.1
通讯作者：
Landis,SC
中科院分区：
文献类型：
--
作者：
Leblanc,GG;Trimmer,BA;Landis,SC
神经肽 Y (NPY) 广泛分布于交感神经系统中，与去甲肾上腺素共存。我们在这里报告，NPY 免疫反应性神经元在大鼠的三个颅副交感神经节（耳、蝶腭和睫状神经节）中也很丰富。对耳神经节中存在的免疫反应物质的高效液相色谱分析表明，该物质与猪 NPY 非常相似，并且与大鼠交感神经元中存在的 NPY 样免疫反应物质没有区别。这些发现提出了 NPY 在副交感神经和交感神经系统中充当神经调节剂的可能性。与在交感神经元中观察到的相反，颅副交感神经节中的 NPY 免疫反应性神经元不包含可检测的儿茶酚胺或酪氨酸羟化酶 (EC 1.14.16.2) 免疫反应性，并且许多确实包含血管活性肠肽和/或胆碱乙酰转移酶 (EC 2.3.1.6) 的免疫反应性。这些发现表明，在自主神经元中，不存在控制 NPY 与去甲肾上腺素、乙酰胆碱或血管活性肠肽共表达的简单规则。此外，虽然功能研究表明 NPY 对外周血管系统的作用与乙酰胆碱和血管活性肠肽的作用相反，但目前的结果提出了这三种物质可能对其他靶组织具有互补作用的可能性。
Neuropeptide Y (NPY) is widely distributed in the sympathetic nervous system, where it is colocalized with norepinephrine. We report here that NPY-immunoreactive neurons are also abundant in three cranial parasympathetic ganglia, the otic, sphenopalatine, and ciliary, in the rat. High-performance liquid chromatographic analysis of the immunoreactive material present in the otic ganglion indicates that this material is very similar to porcine NPY and indistinguishable from the NPY-like immunoreactivity present in rat sympathetic neurons. These findings raise the possibility that NPY acts as a neuromodulator in the parasympathetic as well as the sympathetic nervous system. In contrast to what has been observed for sympathetic neurons, NPY-immunoreactive neurons in cranial parasympathetic ganglia do not contain detectable catecholamines or tyrosine hydroxylase (EC 1.14.16.2) immunoreactivity, and many do contain immunoreactivity for vasoactive intestinal peptide and/or choline acetyltransferase (EC 2.3.1.6). These findings suggest that there is no simple rule governing coexpression of NPY with norepinephrine, acetylcholine, or vasoactive intestinal peptide in autonomic neurons. Further, while functional studies have indicated that NPY exerts actions on the peripheral vasculature which are antagonistic to those of acetylcholine and vasoactive intestinal peptide, the present results raise the possibility that these three substances may have complementary effects on other target tissues.