Hyperspectral Microscopy of Near-Infrared Fluorescence Enables 17-Chirality Carbon Nanotube Imaging.
Hyperspectral Microscopy of Near-Infrared Fluorescence Enables 17-Chirality Carbon Nanotube Imaging.
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DOI：
10.1038/srep14167
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发表时间：
2015-09-21
影响因子：
4.6
通讯作者：
Heller DA
中科院分区：
文献类型：
--
作者：
Roxbury D;Jena PV;Williams RM;Enyedi B;Niethammer P;Marcet S;Verhaegen M;Blais-Ouellette S;Heller DA
单壁碳纳米管的固有近红外光致发光（荧光）表现出独特的光稳定性，窄带宽，穿透生物介质，环境敏感性，以及颜色的多样性和范围。利用这个大家族的荧光团的生物医学应用将需要单个（n，m）纳米管物种的荧光的光谱和空间分辨率及其在活细胞和组织内的调制，这是当前显微镜方法不可能的。我们提出了一个宽视场的高光谱方法来空间描绘和光谱测量单纳米管荧光在生活系统中。这种方法解决了多达17个不同的（n，m）物种（手性）与单纳米管的空间分辨率在活的哺乳动物细胞，鼠组织离体，和斑马鱼内皮在体内。我们预计，这种方法将有助于多路复用纳米管成像在生物医学应用中，同时使深组织光学渗透，并在体内的单分子分辨率。
The intrinsic near-infrared photoluminescence (fluorescence) of single-walled carbon nanotubes exhibits unique photostability, narrow bandwidth, penetration through biological media, environmental sensitivity, and both chromatic variety and range. Biomedical applications exploiting this large family of fluorophores will require the spectral and spatial resolution of individual (n,m) nanotube species’ fluorescence and its modulation within live cells and tissues, which is not possible with current microscopy methods. We present a wide-field hyperspectral approach to spatially delineate and spectroscopically measure single nanotube fluorescence in living systems. This approach resolved up to 17 distinct (n,m) species (chiralities) with single nanotube spatial resolution in live mammalian cells, murine tissues ex vivo, and zebrafish endothelium in vivo. We anticipate that this approach will facilitate multiplexed nanotube imaging in biomedical applications while enabling deep-tissue optical penetration, and single-molecule resolution in vivo.