Anthracene-DNA conjugates as building blocks of designed DNA structures constructed by photochemical reactions
Anthracene-DNA conjugates as building blocks of designed DNA structures constructed by photochemical reactions
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DOI：
10.1039/b821869b
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发表时间：
2009-01-01
影响因子：
3.2
通讯作者：
Jyo, Akinori
中科院分区：
文献类型：
--
作者：
Mukae, Motoko;Ihara, Toshihiro;Jyo, Akinori
我们提出了通过蒽环加成实现蒽-ODN（寡脱氧核糖核苷酸）缀合物的光化学连接。通过将 ODN 的 5'-或 3'-末端连接至蒽基团来合成蒽-DNA 缀合物。缀合物的序列被设计为与目标 ODN 上的相邻位点杂交，并且它们的蒽单元彼此面对。当由两个缀合物和靶标组成的三元双链体形成时，缀合物可以通过光照射二聚化。二聚化效率受蒽基团的取代位置和模板序列中的一碱基置换的影响。此外，还证明双链体的加帽和三链体结构中第三链的连接也可能是光化学连接的目标。
We present the photochemical ligation of anthracene-ODN (oligodeoxyribonucleotide) conjugates through cycloaddition of anthracenes. Anthracene-DNA conjugates were synthesized by linking the 5'-or 3'-end of ODNs to the anthracene group. The sequences of the conjugates were designed to hybridize to neighboring sites on the target ODN with their anthracene units facing each other. When the ternary duplex consisting of the two conjugates and the target forms, the conjugates can be dimerized by light irradiation. The dimerization efficiency was affected by the substitution position of the anthracene group and by a one-base displacement in the template sequence. Furthermore, it is demonstrated that the capping of the duplex and connecting third strands in a triplex structure could also be the target of the photochemical ligation.