SELF-ASSOCIATION OF DAUNORUBICIN
SELF-ASSOCIATION OF DAUNORUBICIN
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DOI：
10.1016/0014-5793(74)80322-4
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发表时间：
1974-01-01
期刊：
影响因子：
3.5
通讯作者：
SICARD, P
中科院分区：
文献类型：
--
作者：
BARTHELE.V;MAURIZOT, JC;SICARD, P
柔红霉素是一种蒽环类抗生素，分离自具有细胞毒性和抗有丝分裂活性的Sfreptumerium Caeruleorubidus的培养物。目前，它被用于治疗急性白血病[1]。已经积累了大量的证据，这些证据与DNA的嵌入复合物的存在一致[2]，其导致酶促RNA和DNA合成的抑制[3，4]。对DNA-柔红霉素复合物的物理化学性质的研究[5]使我们对该分子在溶液中的行为进行了初步研究。可以预期，由于其平面芳环，分子之间可能存在强相互作用，如许多化合物如放线菌素[6]、原黄素[7]、嘌呤衍生物[8-IO]或吖啶橙子[1]所示。本文报道了通过各种物理化学方法：紫外和可见吸收，圆二色性（CD）和核磁共振（NMR）研究抗生素浓度的影响的研究结果。从这些不同的方法所产生的结果之间的比较，提出了柔红霉素的自缔合模型。
Daunorubicin is an antibiotic of the anthracycline group isolated from cultures of Sfreptumyces Caeruleorubidus with cytoxic and antimitotic activities. Presently it is used in the treatment of acute leukemia [1]. A considerable body of evidence has been accumulated which is in agreement with the existence of an intercalative complex with the DNA [2], which results in inhibition of both enzymatic RNA and DNA synthesis [3, 4]. The study of the physico-chemical properties of the DNA-Daunorubicin complex [5] led us to a preliminary study of the behaviour of this molecule in solution. It could be expected that, due to its planar aromatic ring, strong interactions between molecules could exist as already shown for many compounds such as actinomycin [6], proflavine [7], purine derivatives [8-IO] or acridine orange [1 l]. This paper reports results of a study on the effects of the antibiotic concentration investigated by various physicochemical methods: ultraviolet and visible absorption, circular dichroism (CD) and nuclear magnetic resonance (NMR). From the comparison between the results yielded by these different methods a model for the self-association of Daunorubicin is proposed.