Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis System
Production of Immunizing Antigen Proteoliposome Using Cell-Free Protein Synthesis System
复制标题
利用无细胞蛋白质合成系统生产免疫抗原脂蛋白体
DOI：
10.1007/978-1-4939-8802-0_7
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Takeda Hiroyuki
中科院分区：
文献类型：
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作者：
Zhou Wei;Takeda Hiroyuki
特异性识别膜蛋白的抗体是膜蛋白功能分析、诊断和治疗的重要工具。然而，开发针对膜蛋白的抗体仍然是一个很大的挑战，因为膜蛋白的大规模生产是困难的。最近，我们开发了一种高效的无细胞生产方法的蛋白脂质体抗原使用无细胞蛋白合成方法与脂质体和透析杯。在这里，我们介绍了实用和有效的集成程序，以产生大量的蛋白脂质体抗原的抗膜蛋白抗体的发展。
Antibodies specifically recognizing integral membrane protein are essential tool for functional analysis, diagnosis, and therapeutics targeting membrane proteins. However, development of antibodies against membrane protein remains a big challenge, because mass production of membrane protein is difficult. Recently, we developed a highly efficient cell-free production method of proteoliposome antigen using cell-free protein synthesis method with liposome and dialysis cup. Here we introduce practical and efficient integrated procedures to produce large amount of proteoliposome antigen for anti-membrane protein antibody development.