Prevotella intermedia and Prevotella nigrescens serotypes, ribotypes and binding characteristics.
Prevotella intermedia and Prevotella nigrescens serotypes, ribotypes and binding characteristics.
复制标题
中间普雷沃菌和黑质普雷沃菌血清型、核糖型和结合特征。
作者：
Gunnar Dahlén;Jolene Johnson;R. Gmür
应用基因组DNA指纹和rRNA基因探针对模式菌株和62株中间普氏杆菌和黑色普氏杆菌临床分离株进行了基因分型。用单抗对菌株进行了进一步的血清学分型，并对其进行了SDS-PAGE、酶活性、溶血和血凝以及与链球菌和放线菌的共聚集等特性的研究。中间疫霉和黑色疫霉具有明显的核型模式，分别对应于先前定义的血清型I和II/III。中间假单胞菌和偏头痛假单胞菌在溶血、血凝、与链球菌和放线菌的共聚集以及氨基肽和脂肪酶的表达方面没有明显的表型差异。
Type strains and 62 clinical isolates of Prevotella intermedia and Prevotella nigrescens were typed with the use of genomic DNA fingerprints and rRNA gene probes. The strains were further serotyped with monoclonal antibodies and characterized with SDS-PAGE, enzymatic activities, hemolysis and hemagglutination and coaggregation with Streptococcus and Actinomyces spp. P. intermedia and P. nigrescens were found to have distinct ribotype patterns which correspond to previously defined serotyupes I and II/III, respectively. No clear phenotypic difference related to hemolysis, hemagglutination and coaggregation with streptococcus and actinomyces species, or expression of aminopeptides and lipase was found between P. intermedia and P. migrescens.