Demonstration of anticollagen antibodies in rheumatoid arthritis synovial fluids by 14C-radioimmunoassay.
Demonstration of anticollagen antibodies in rheumatoid arthritis synovial fluids by 14C-radioimmunoassay.
复制标题
通过 14C 放射免疫测定法证实类风湿性关节炎滑液中的抗胶原抗体。
作者：
J. Menzel;C. Steffen;G. Kolarz;M. Kojer;J. Smolen
用放射免疫分析法测定了27例类风湿关节炎（RA）患者和17例正常人关节液中抗胶原抗体的含量。将天然或变性状态的体外标记的人I型14 C-胶原用作抗原。被动血凝用于比较。在对照滑液的基础上评价放射免疫测定中定义阳性结果的参数。20例RA患者（74%）的滑液显示变性胶原抗体; 8例RA患者（30%）的滑液也显示天然胶原抗体。创伤后积液的对照液呈阴性;在其他对照中，1例银屑病关节炎患者的滑液呈阳性反应。抑制实验表明，变性胶原蛋白的抗体与天然胶原蛋白发生交叉反应。与人变性III型胶原蛋白的抑制显示抗I型胶原蛋白抗体与III型胶原蛋白的强交叉反应性。
Twenty-seven synovial fluids from rheumatoid arthritis (RA) patients and 17 synovial fluids from controls were investigated in a new radioimmunoassay for anticollagen antibodies. In vitro labeled human 14C-collagen of type I in native or denatured state was used as antigen. Passive hemagglutination was used in comparison. Parameters for defining positive results in radioimmunoassay were evaluated on the basis of control synovial fluids. Synovial fluids from 20 RA patients (74%) showed antibodies to denatured collagen; synovial fluids from 8 RA patients (30%) also demonstrated antibodies to native collagen. Control fluids of posttraumatic effusions were negative; among the other controls synovial fluid from 1 psoriatic arthritis patient reacted positively. Inhibition experiments showed that antibodies to denatured collagen cross-reacted with native collagen. Inhibition with human denatured type III collagen displayed strong cross-reactivity of anti-type I collagen antibodies with type III collagen.