WIRED ENZYME ELECTRODES FOR AMPEROMETRIC DETERMINATION OF GLUCOSE OR LACTATE IN THE PRESENCE OF INTERFERING SUBSTANCES
WIRED ENZYME ELECTRODES FOR AMPEROMETRIC DETERMINATION OF GLUCOSE OR LACTATE IN THE PRESENCE OF INTERFERING SUBSTANCES
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DOI：
10.1021/ac00087a008
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发表时间：
1994-08-01
影响因子：
7.4
通讯作者：
HELLER, A
中科院分区：
文献类型：
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作者：
OHARA, TJ;RAJAGOPALAN, R;HELLER, A
葡萄糖氧化酶（GOX）或乳酸氧化酶（LOX）固定在锇基三维氧化还原水凝胶中，该水凝胶将酶的氧化还原中心电连接到电极上。酶“接线”水凝胶是由聚（1-乙烯基咪唑）（PVI）与Os-（4,4'-二甲酰基）(2)Cl（称为PVI15-dmeOs）与聚（乙二醇）二甘油酯醚（peg）交联而成。葡萄糖和乳酸传感器的典型极限电流密度分别为250和500 μ A/cm(2)。当电极保持在200 mV （SCE）时，抗坏血酸盐、尿酸盐、对乙酰氨基酚和l -半胱氨酸的电氧化产生的电流可以忽略不计。当使用Nafion薄膜时，线性范围从6到30 mM葡萄糖和4到7 mM乳酸扩展。成胶聚合物的氧化还原电位为95 mV （SCE）。在牛血清中进行的葡萄糖和乳酸测量与磷酸盐缓冲液中的底物校准相关良好。
Glucose oxidase (GOX) or lactate oxidase (LOX) were immobilized in an osmium-based three-dimensional redox hydrogel that electrically connected the enzyme's redox centers to electrodes. The enzyme ''wiring'' hydrogel was formed by cross-linking poly(1-vinylimidazole) (PVI) complexed with Os-(4,4'-dimethylbpy)(2)Cl (termed PVI15-dmeOs) with poly(ethylene glycol) diglycidyl ether (peg). Glucose and lactate sensors exhibited typical limiting current densities of 250 and 500 mu A/cm(2), respectively. When the electrodes were poised at 200 mV (SCE), the currents resulting from electrooxidation of ascorbate, urate, acetaminophen, and L-cysteine were negligible. When a Nafion film was employed, the linear range was extended from 6 to 30 mM glucose and from 4 to 7 mM lactate. The redox potential of the gel-forming polymer was 95 mV (SCE). Glucose and lactate measurements performed in bovine calf serum correlated well with a substrate calibration in phosphate buffer.