ESHRE PGD consortium best practice guidelines for amplification-based PGD
ESHRE PGD consortium best practice guidelines for amplification-based PGD
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DOI：
10.1093/humrep/deq231
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发表时间：
2011-01-01
影响因子：
6.1
通讯作者：
Harper, J. C.
中科院分区：
文献类型：
--
作者：
Harton, G. L.;De Rycke, M.;Harper, J. C.
2005年，欧洲人类生殖和胚胎学学会（ESHRE） PGD联合会出版了一套最佳PGD实践指南，为潜在的、现有的和刚刚开始的PGD计划提供信息、支持和指导。在随后的几年里，我们看到了许多新技术的引入以及现有技术的发展。此外，鉴于ESHRE最近关于如何编写和制定实践指南的建议，该联盟认为修订和更新PGD指南是及时的。与原始出版物中涵盖所有PGD的一份文件不同，这些指南被分成四个新文件，分别适用于PGD计划的不同方面，即PGD中心的组织，基于荧光原位杂交的检测，基于扩增的检测以及用于PGD/植入前遗传筛查的极体和胚胎活检。在这里，我们更新了与基于扩增的PGD相关的部分。本指南涵盖的主题包括基于扩增的PGD测试的纳入/排除标准、测试的临床前验证、基于扩增的测试方法、用于分析的细胞管、当地试管婴儿中心和运输PGD中心的建立、质量控制/质量保证和未移植胚胎的诊断确认。
In 2005, the European Society for Human Reproduction and Embryology (ESHRE) PGD Consortium published a set of Guidelines for Best Practice PGD to give information, support and guidance to potential, existing and fledgling PGD programmes. The subsequent years have seen the introduction of a number of new technologies as well as the evolution of current techniques. Additionally, in light of recent advice from ESHRE on how practice guidelines should be written and formulated, the Consortium believed it was timely to revise and update the PGD guidelines. Rather than one document that covers all of PGD, as in the original publication, these guidelines are separated into four new documents that apply to different aspects of a PGD programme, i.e. Organization of a PGD centre, fluorescence in situ hybridization-based testing, Amplification-based testing and Polar Body and Embryo Biopsy for PGD/preimplantation genetic screening. Here, we have updated the sections that pertain to amplification-based PGD. Topics covered in this guideline include inclusion/exclusion criteria for amplification-based PGD testing, preclinical validation of tests, amplification-based testing methods, tubing of cells for analysis, set-up of local IVF centre and Transport PGD centres, quality control/quality assurance and diagnostic confirmation of untransferred embryos.