Sebox regulates mesoderm formation in early amphibian embryos
Sebox regulates mesoderm formation in early amphibian embryos
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Sebox 调节早期两栖动物胚胎中胚层的形成
DOI：
10.1002/dvdy.24323
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发表时间：
2015-11-01
影响因子：
2.5
通讯作者：
Tao, Qinghua
中科院分区：
文献类型：
--
作者：
Chen, Geng;Tan, Renbo;Tao, Qinghua
背景：Mix/Bix基因是脊椎动物胚胎发育过程中中胚层形成的重要调节因子。Sebox是该基因家族的另一个成员，在斑马鱼早期胚胎发生过程中参与了内胚层的形成。然而，目前尚不清楚Sebox在非洲爪哇早期胚胎中是否扮演着独特的角色。结果：在这项研究中，我们提供了证据，在非洲爪哇早期胚胎发育过程中，SEBOX是中胚层形成所必需的。在原肠形成过程中，SEBOX在退化的中胚层中动态表达。它被Nodal/Activin信号激活，并受Wnt/β-catenin合子信号的调节。Sebox的过表达扰乱了收敛伸展过程中的运动，并抑制了Nodal/Activin信号诱导的中胚层基因的表达，但不抑制内胚层基因的表达。用特定的吗啉基耗尽SEBOX会增加非经典的WNT5A、WNT5B和WNT11B的表达。Sebox的缺失还以Wnt11B依赖的方式上调PCDH8.2的表达，PCDH8.2是一种旁轴中胚层特异性的原钙粘附素。Sebox变种显示头部和脊索发育减慢。结论：Sebox是Mix/Bix基因家族中一个独特的成员，其功能位于Nodal/Activin信号的下游，是非洲爪哇中胚层正常发育和正常表达非规范Wnt配体所必需的。发展动力学244：1415-1426,2015。©2015 Wiley期刊，Inc.
Background: Mix/Bix genes are important regulators of mesendoderm formation during vertebrate embryogenesis. Sebox, an additional member of this gene family, has been implicated in endoderm formation during early embryogenesis in zebrafish. However, it remains unclear whether Sebox plays a unique role in early Xenopus embryos. Results: In this study, we provide evidence that Sebox is uniquely required for the formation of mesoderm during early Xenopus embryogenesis. Sebox is dynamically expressed in the involuted mesoderm during gastrulation. It is activated by Nodal/Activin signaling and modulated by zygotic Wnt/β‐catenin signaling. Overexpression of Sebox perturbs movements during convergent extension and inhibits the expression of mesodermal, but not endodermal, genes induced by Nodal/Activin signaling. Depletion of Sebox using a specific morpholino increases the expression of noncanonical wnt5a, wnt5b, and wnt11b. Depletion of Sebox also up‐regulates the expression of pcdh8.2, a paraxial mesoderm‐specific protocadherin, in a Wnt11B‐dependent manner. Sebox morphants display reduced development of the head and notochord. Conclusions: Our findings illustrate that Sebox, a unique member of the Mix/Bix gene family, functions downstream of Nodal/Activin signaling and is required for the proper expression of noncanonical Wnt ligands and the normal development of mesoderm in Xenopus. Developmental Dynamics 244:1415–1426, 2015. © 2015 Wiley Periodicals, Inc.