Mammalian cell cultivation using nutrients extracted from microalgae
Mammalian cell cultivation using nutrients extracted from microalgae
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DOI：
10.1002/btpr.2941
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发表时间：
2019-12-09
影响因子：
2.9
通讯作者：
Shimizu, Tatsuya
中科院分区：
文献类型：
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作者：
Okamoto, Yuta;Haraguchi, Yuji;Shimizu, Tatsuya
哺乳动物细胞已被用于各种研究领域。最近，培养细胞已被用作“培养肉”的细胞来源。“细胞培养需要含有营养物质的培养基，其中葡萄糖和氨基酸是必需的。这些营养素通常来源于谷物或异养微生物，它们也需要来源于谷物的各种营养素。粮食种植需要大量的化肥和农用化学品，这可能导致温室气体排放和环境污染。此外，粮食生产受环境变化的影响很大。相比之下，微藻利用太阳能、水和无机物高效合成各种营养物质，广泛应用于能源领域。在这项研究中，我们的目的是将微藻提取的营养物质应用于哺乳动物细胞培养的培养基中。葡萄糖是有效地从Chlorococcum littorale或Arthrospira platensis使用硫酸提取，而18的20个蛋白质氨基酸是有效地从小球藻使用盐酸提取。我们进一步研究了藻类提取物中存在的营养物质是否可以用于哺乳动物细胞培养。尽管几乎所有的C2C12小鼠成肌细胞在无葡萄糖和氨基酸的培养基中培养期间死亡，但通过将藻类提取物添加到营养缺乏的培养基中来挽救细胞死亡。这表明藻类提取物中存在的营养物质可用于哺乳动物细胞培养。这项研究是建立一种新的细胞培养系统的第一步，该系统可以减少环境负荷，并不受环境变化的影响。
Mammalian cells have been used in various research fields. More recently, cultured cells have been used as the cell source of "cultured meat." Cell cultivation requires media containing nutrients, of which glucose and amino acids are the essential ones. These nutrients are generally derived from grains or heterotrophic microorganisms, which also require various nutrients derived from grains. Grain culture, in turn, requires many chemical fertilizers and agrochemicals, which can cause greenhouse gas emission and environmental contamination. Furthermore, grain production is greatly influenced by environmental changes. In contrast, microalgae efficiently synthesize various nutrients using solar energy, water, and inorganic substances, which are widely used in the energy sector. In this study, we aimed to apply nutrients extracted from microalgae in the culture media for mammalian cell cultivation. Glucose was efficiently extracted from Chlorococcum littorale or Arthrospira platensis using sulfuric acid, whereas 18 of the 20 proteinogenic amino acids were efficiently extracted from Chlorella vulgaris using hydrochloric acid. We further investigated whether nutrients present in the algal extracts could be used in mammalian cell cultivation. Although almost all C2C12 mouse myoblasts died during cultivation in a glucose- and amino acid-free medium, the cell death was rescued by adding algal extract(s) into the nutrient-deficient media. This indicates that nutrients present in algal extracts can be used for mammalian cell cultivation. This study is the first step toward the establishment of a new cell culture system that can reduce environmental loads and remain unaffected by the impact of environmental changes.