Enzymatic hydrolysis of PTT polymers and oligomers
Enzymatic hydrolysis of PTT polymers and oligomers
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DOI：
10.1016/j.jbiotec.2008.02.015
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发表时间：
2008-05-20
影响因子：
4.1
通讯作者：
Guebitz, G. M.
中科院分区：
文献类型：
--
作者：
Eberl, A.;Heumann, S.;Guebitz, G. M.
用来自嗜热菌、桔青霉、褐色嗜热双歧杆菌和梨腐镰刀菌的聚酯酶处理线型芳香族聚酯聚对苯二甲酸丙二醇酯（PTT）的低聚物和聚合物（薄膜、织物）。发现来自T fusca的角质酶从PTT材料释放最高量的水解产物，并且根据RP-HPLC-UV检测能够打开和水解环状PTT二聚体。相比之下，来自Tlanaprosus的脂肪酶也显示出对PTT纤维和对对苯二甲酸双（3-羟丙基）酯（BHPT）的活性，但不能水解聚合物膜、对苯二甲酸单（3-羟丙基）酯（MHPT）或PTT的环状二聚体。作为对照，使用用氯化汞抑制的酶。表面亲水性的变化进行了研究与接触角的测量和结晶度的变化与DSC测定。(C)2008 Elsevier B.V.保留所有权利。
Oligomers and polymers (film, fabrics) of the linear aromatic polyester poly(trimethylene terephthalate) (PTT) were treated with polyesterases from Thermomyces lanuginosus, Penicillium citrinum, Thermobifida fusca and Fusarium solani pisi. The cutinase from T fusca was found to release the highest amounts of hydrolysis products from PTT materials and was able to open and hydrolyse a cyclic PTT dimer according to RP-HPLC-UV detection. In contrast, the lipase from T lanuginosus also showed activity on the PTT fibres and on bis(3-hydroxypropyl) terephthalate (BHPT) but was not able to hydrolyse the polymer film, mono(3-hydroxypropyl) terephthalate (MHPT) nor the cyclic dimer of PTT. As control enzymes inhibited with mercury chloride were used. Surface hydrophilicity changes were investigated with contact angle measurements and the degree of crystallinity changes were determined with DSC. (C) 2008 Elsevier B.V. All rights reserved.