Virgibacillus sediminis sp nov., a moderately halophilic bacterium isolated from a salt lake in China
Virgibacillus sediminis sp nov., a moderately halophilic bacterium isolated from a salt lake in China
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Virgibacillus sediminis sp.
DOI：
10.1099/ijs.0.003624-0
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发表时间：
2009-08-01
影响因子：
2.8
通讯作者：
Peng, Qian
中科院分区：
文献类型：
--
作者：
Chen, Yi-Guang;Cui, Xiao-Long;Peng, Qian
从柴达木盆地盐湖沉积物中分离到一株革兰氏阳性、中度嗜盐、耐碱、严格好氧、氧化酶和过氧化氢酶阳性的杆状细菌YIM kkny 3 T。细胞通过周生鞭毛运动，形成椭圆形的内生孢子，位于近端膨大的孢子囊内。在1-20%（w/v）总盐（最佳，5-10%）和pH 6.0-10.5（最佳，pH 7.5-8.0）和10-55 ℃（最佳，35-40 ℃）下发生生长。它不能以NaCl作为唯一的盐生长。meso-二氨基庚二酸存在于细胞壁肽聚糖中。该菌株含有甲基萘醌7（MK-7）作为主要的呼吸醌和二磷脂酰甘油，磷脂酰甘油，磷脂酰乙醇胺和一个身份不明的磷脂作为极性脂质。主要的细胞脂肪酸是anteiso-C15：0和anteiso-C17：0。DNA G+C含量为40.9mol%。基于16 S rRNA基因序列的系统发育分析表明，菌株YIM kkny 3 T属于芽枝芽孢杆菌属，与橄榄芽枝芽孢杆菌（97.1%相似性）、海洋芽枝芽孢杆菌（97.0%）和可可芽枝芽孢杆菌（96.8%）模式菌株的亲缘关系最近。YIM kkny 3 T菌株与模式菌株Olivis、V. marismortui和V. kekensis的DNA-DNA相关性分别为12.4%、10.6%和15.7%。系统发育分析、基因型数据、表型特征和化学分类学差异的结合表明，菌株YIM kkny 3 T代表了Virgibacillus属的一个新种，建议将其命名为Virgibacillus sediminis sp. nov.。模式菌株是YIM kkny 3 T（=CCTCC AA 207023 T =DSM 19797 T =KCTC 13193 T）。
A Gram-positive, moderately halophilic, alkalitolerant, strictly aerobic, oxidase- and catalase-positive, rod-shaped bacterium, strain YIM kkny3T, was isolated from a sediment sample collected from a salt lake in the Qaidam Basin of north-west China. Cells were motile by means of peritrichous flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. Growth occurred with 1-20% (w/v) total salts (optimum, 5-10%) and at pH 6.0-10.5 (optimum, pH 7.5-8.0) and 10-55 degrees C (optimum, 35-40 degrees C). It was unable to grow with NaCl as the only salt. meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The strain contained menaquinone 7 (MK-7) as the predominant respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid as polar lipids. The major cellular fatty acids were anteiso-C15:0 and anteiso-C17:0. The DNA G+C content was 40.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM kkny3T belonged to the genus Virgibacillus, and was most closely related to the type strains of Virgibacillus olivae (97.1% similarity), Virgibacillus marismortui (97.0%) and Virgibacillus kekensis (96.8%). Levels of DNA-DNA relatedness between strain YIM kkny3T and the type strains of V. olivae, V. marismortui and V. kekensis were 12.4, 10.6 and 15.7%, respectively. The combination of phylogenetic analysis, genotypic data, phenotypic characteristics and chemotaxonomic differences indicated that strain YIM kkny3T represents a novel species of the genus Virgibacillus, for which the name Virgibacillus sediminis sp. nov. is proposed. The type strain is YIM kkny3T (=CCTCC AA 207023T=DSM 19797T=KCTC 13193T).