Dethiosulfatarculus sandiegensis gen. nov., sp nov., isolated from a methanogenic paraffin-degrading enrichment culture and emended description of the family Desulfarculaceae
Dethiosulfatarculus sandiegensis gen. nov., sp nov., isolated from a methanogenic paraffin-degrading enrichment culture and emended description of the family Desulfarculaceae
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DOI：
10.1099/ijsem.0.000864
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发表时间：
2016-03-01
影响因子：
2.8
通讯作者：
Suflita, Joseph M.
中科院分区：
文献类型：
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作者：
Davidova, Irene A.;Wawrik, Boris;Suflita, Joseph M.
从一个产甲烷菌群中分离到一株能降解长链烷烃的中温δ-变形杆菌，命名为SPRT。细胞是能动的，弧菌形，并单独出现，成对或成簇。菌株SPRT不代谢碳氢化合物，但生长发酵丙酮酸和草酰乙酸和自养与H-2和CO2。硫代硫酸盐作为末端电子受体，但硫酸盐或亚硫酸盐没有。该生物体需要至少10克NaCl l（-1）和少量的酵母提取物（0.001%）的生长。在30至37摄氏度和6.0至7.2的pH范围内观察到最佳生长。SPRT基因组DNA的G + C含量为52.02mol%。基于16 S rRNA基因序列分析，菌株SPRT与先前描述的三角变形菌不同，表现出与Desulfoculus baarsii DSM 2075（T）和Desulfocarbo indianensis SCBMT最接近的联系，它们各自的16 S基因序列之间只有91%的相似性。计算机模拟基因组比较支持菌株SPRT与脱硫碳印度SCBMT和脱硫菌DSM 2075（T）之间的区别。基于生理差异，以及系统发育和基因组比较，我们建议将SPRT分类为一个新属的一个新种的模式菌株（=DSM 100305（T）=JCM 30857（T）），命名为Dethiosulfatarculus sandiegen. nov.，sp. Nov.
A mesophilic deltaproteobacterium, designated strain SPRT, was isolated from a methanogenic consortium capable of degrading long-chain paraffins. Cells were motile, vibrio-shaped, and occurred singly, in pairs or in clusters. Strain SPRT did not metabolize hydrocarbons but grew fermentatively on pyruvate and oxaloacetate and autotrophically with H-2 and CO2. Thiosulfate served as a terminal electron acceptor, but sulfate or sulfite did not. The organism required at least 10 g NaCl l(-1) and a small amount of yeast extract (0.001%) for growth. Optimal growth was observed between 30 and 37 degrees C and a pH range from 6.0 to 7.2. The DNA G + C content of SPRT's genome was 52.02 mol%. Based on 16S rRNA gene sequence analysis, strain SPRT was distinct from previously described Deltaproteobacteria, exhibiting the closest affiliation to Desulfarculus baarsii DSM 2075(T) and Desulfocarbo indianensis SCBMT, with only 91% similarity between their respective 16S gene sequences. In silico genome comparison supported the distinctiveness between strain SPRT and both Desulfocarbo indianensis SCBMT and Desulfarculus baarsii DSM 2075(T). Based on physiological differences, as well as phylogenetic and genomic comparisons, we propose to classify SPRT as the type strain (=DSM 100305(T)=JCM 30857(T)) of a novel species of a new genus with the name Dethiosulfatarculus sandiegensis gen. nov., sp. nov.